Detailed information of TA system TA020035

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/-
• Location: 2067258..2068089
• Replicon: chromosome
• Accession: CP009789
• Organism: Escherichia coli K-12 strain ER3413

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: ER3413_2068
• Protein ID: AIZ51817.1
• Coordinates: 2067715..2068089 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: ER3413_2067
• Protein ID: AIZ51816.1
• Coordinates: 2067258..2067626 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER3413_2064	2064925..2066457	+	1533	AIZ51813.1	CP4-44 prophage; predicted membrane protein	-
ER3413_2065	2066454..2066900	+	447	AIZ51814.1	CP4-44 prophage; predicted DNA repair protein	-
ER3413_2066	2066963..2067184	+	222	AIZ51815.1	CP4-44 prophage; putative protein	-
ER3413_2067	2067258..2067626	+	369	AIZ51816.1	CP4-44 prophage; cytoskeleton bundling-enhancing factor A; CbtA antitoxin	Antitoxin
ER3413_2068	2067715..2068089	+	375	AIZ51817.1	CP4-44 prophage; toxin of the YeeV-YeeU toxin-antitoxin system	Toxin
ER3413_2070	2069178..2069507	-	330	AIZ51818.1	conserved protein, UPF0265 family	-
ER3413_2071	2069679..2070737	-	1059	AIZ51819.1	inner membrane protein, FUSC family	-
ER3413_2073	2070935..2071408	-	474	AIZ51820.1	DNA gyrase inhibitor	-
ER3413_2074	2071527..2072693	-	1167	AIZ51821.1	D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 6b)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T20035 AIZ51817.1 CP009789:2067715-2068089 [Escherichia coli K-12]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T20035 CP009789:2067715-2068089 [Escherichia coli K-12]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT20035 AIZ51816.1 CP009789:2067258-2067626 [Escherichia coli K-12]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT20035 CP009789:2067258-2067626 [Escherichia coli K-12]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA