Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1854686..1855183 Replicon chromosome
Accession NZ_CP073809
Organism Macrococcus equipercicus strain Epi0143-OL

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag KFV11_RS09850 Protein ID WP_149459118.1
Coordinates 1854686..1855039 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag KFV11_RS09855 Protein ID WP_254249835.1
Coordinates 1855040..1855183 (-) Length 48 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV11_RS09825 (KFV11_09835) 1849761..1851905 - 2145 WP_254249833.1 Tex family protein -
KFV11_RS09830 (KFV11_09840) 1851973..1852749 - 777 WP_149459114.1 RNA polymerase sigma factor SigB -
KFV11_RS09835 (KFV11_09845) 1852724..1853200 - 477 WP_149459115.1 anti-sigma B factor RsbW -
KFV11_RS09840 (KFV11_09850) 1853197..1853523 - 327 WP_254249834.1 anti-sigma factor antagonist -
KFV11_RS09845 (KFV11_09855) 1853606..1854613 - 1008 WP_149459117.1 PP2C family protein-serine/threonine phosphatase -
KFV11_RS09850 (KFV11_09860) 1854686..1855039 - 354 WP_149459118.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFV11_RS09855 (KFV11_09865) 1855040..1855183 - 144 WP_254249835.1 antitoxin MazE Antitoxin
KFV11_RS09860 (KFV11_09870) 1855249..1856394 - 1146 WP_254249836.1 alanine racemase -
KFV11_RS09865 (KFV11_09875) 1856490..1856837 - 348 WP_149459318.1 holo-ACP synthase -
KFV11_RS09870 (KFV11_09880) 1857007..1858509 - 1503 WP_149459121.1 DEAD/DEAH box helicase -
KFV11_RS09875 (KFV11_09885) 1858701..1860044 - 1344 WP_254249837.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 12870.81 Da        Isoelectric Point: 7.2048

>T200333 WP_149459118.1 NZ_CP073809:c1855039-1854686 [Macrococcus equipercicus]
MKRGDVYLADLSPVKGSEQGGKRPVVIIQNDVGNKFSPTVIVAAITAKINKARIPTHVEIGRTNHQLDKDSVILLEQIRT
IDKNRLIDKLTVLSPDKMDEVDEALEISLGLSGRSQE

Download         Length: 354 bp

>T200333 NZ_CP097831:1797689-1798096 [Shigella flexneri]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 48 a.a.        Molecular weight: 5450.00 Da        Isoelectric Point: 3.4961

>AT200333 WP_254249835.1 NZ_CP073809:c1855183-1855040 [Macrococcus equipercicus]
MKKFEQLLAEGYQSMAELNLMISYEQFELECEASEQSDCFFASEDGE

Download         Length: 144 bp

>AT200333 NZ_CP097831:1797442-1797708 [Shigella flexneri]
ATGGACATTAACAACAAAGCCCGAATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References