Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1995713..1996204 Replicon chromosome
Accession NZ_CP073808
Organism Macrococcus canis strain IMD0709

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag KFV10_RS10580 Protein ID WP_086043355.1
Coordinates 1995713..1996063 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag KFV10_RS10585 Protein ID WP_101495293.1
Coordinates 1996064..1996204 (-) Length 47 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV10_RS10555 (KFV10_10555) 1990821..1992956 - 2136 WP_254253793.1 Tex family protein -
KFV10_RS10560 (KFV10_10560) 1993033..1993800 - 768 WP_133420462.1 RNA polymerase sigma factor SigB -
KFV10_RS10565 (KFV10_10565) 1993805..1994248 - 444 WP_086043352.1 anti-sigma B factor RsbW -
KFV10_RS10570 (KFV10_10570) 1994253..1994576 - 324 WP_086043353.1 anti-sigma factor antagonist -
KFV10_RS10575 (KFV10_10575) 1994657..1995664 - 1008 WP_086043354.1 PP2C family protein-serine/threonine phosphatase -
KFV10_RS10580 (KFV10_10580) 1995713..1996063 - 351 WP_086043355.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFV10_RS10585 (KFV10_10585) 1996064..1996204 - 141 WP_101495293.1 antitoxin MazE Antitoxin
KFV10_RS10590 (KFV10_10590) 1996266..1997408 - 1143 WP_086043356.1 alanine racemase -
KFV10_RS10595 (KFV10_10595) 1997434..1997790 - 357 WP_254253794.1 holo-ACP synthase -
KFV10_RS10600 (KFV10_10600) 1997732..1998253 - 522 WP_236365977.1 PH domain-containing protein -
KFV10_RS10605 (KFV10_10605) 1998237..1999748 - 1512 WP_254253795.1 PH domain-containing protein -
KFV10_RS10610 (KFV10_10610) 1999738..2000214 - 477 WP_086043360.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12705.88 Da        Isoelectric Point: 9.9423

>T200332 WP_086043355.1 NZ_CP073808:c1996063-1995713 [Macrococcus canis]
MKRGDVYLADLSPVKGSEQGGKRPVVIIQNNIGNKHSPTVIVAAITAKINKAKIPTHVEIGKTTHHLEKDSVILLEQIRT
IDKNRLIDHLTVLDKAKMKEVDVALLISLGLSIDSE

Download         Length: 351 bp

>T200332 NZ_CP097831:1596968-1597282 [Shigella flexneri]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 47 a.a.        Molecular weight: 5319.89 Da        Isoelectric Point: 3.8301

>AT200332 WP_101495293.1 NZ_CP073808:c1996204-1996064 [Macrococcus canis]
MSQKLEQMLIEGYQSMAEINLSIASEHYILECEVNDNHQTYTLDGE

Download         Length: 141 bp

>AT200332 NZ_CP097831:1597279-1597695 [Shigella flexneri]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References