Detailed information of TA system TA200327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1202219..1202706
• Replicon: chromosome
• Accession: NZ_CP073805
• Organism: Macrococcus canis strain Epi3241-OR

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KFV08_RS06090
• Protein ID: WP_252455112.1
• Coordinates: 1202491..1202706 (+)
• Length: 72 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KFV08_RS06085
• Protein ID: WP_236365521.1
• Coordinates: 1202219..1202443 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV08_RS06045 (KFV08_06040)	1197492..1197743	+	252	WP_252455606.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KFV08_RS06050 (KFV08_06045)	1197780..1198226	-	447	WP_252455107.1	GNAT family N-acetyltransferase	-
KFV08_RS06055 (KFV08_06050)	1198306..1199046	+	741	WP_252455108.1	hypothetical protein	-
KFV08_RS06060 (KFV08_06055)	1199056..1199946	+	891	WP_252455109.1	phosphotransferase	-
KFV08_RS06065 (KFV08_06060)	1200132..1200617	+	486	WP_252455110.1	NUDIX domain-containing protein	-
KFV08_RS06070 (KFV08_06065)	1200623..1201390	+	768	WP_252455111.1	hypothetical protein	-
KFV08_RS06075 (KFV08_06070)	1201408..1201680	-	273	WP_138071160.1	Txe/YoeB family addiction module toxin	-
KFV08_RS06080 (KFV08_06075)	1201673..1201930	-	258	WP_210151726.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KFV08_RS06085 (KFV08_06080)	1202219..1202443	+	225	WP_236365521.1	DUF6290 family protein	Antitoxin
KFV08_RS06090 (KFV08_06085)	1202491..1202706	+	216	WP_252455112.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KFV08_RS06095 (KFV08_06090)	1202817..1203185	+	369	WP_252455113.1	GntR family transcriptional regulator	-
KFV08_RS06100 (KFV08_06095)	1203185..1204285	+	1101	WP_252455114.1	DUF1648 domain-containing protein	-
KFV08_RS06105 (KFV08_06100)	1204413..1204937	+	525	WP_158554239.1	GNAT family N-acetyltransferase	-
KFV08_RS06110 (KFV08_06105)	1205012..1205542	+	531	WP_252455115.1	DinB family protein	-
KFV08_RS06115 (KFV08_06110)	1205553..1206002	+	450	WP_252455116.1	GNAT family N-acetyltransferase	-
KFV08_RS06120 (KFV08_06115)	1206122..1207339	+	1218	WP_099578034.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-70)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 72 a.a.
• Molecular weight: 8355.73 Da
• Isoelectric Point: 9.8884

>T200327 WP_252455112.1 NZ_CP073805:1202491-1202706 [Macrococcus canis]
MDPQSSRLLLQWINKNIVHCENPRSSGKALTGNYSGIWCYRVGQFRIITKIEDDKLIILVISIDYRKNIYR

Nucleotide

• Length: 216 bp

>T200327 NZ_CP097829:c151831-151556 [Shigella flexneri]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8745.91 Da
• Isoelectric Point: 4.4193

>AT200327 WP_236365521.1 NZ_CP073805:1202219-1202443 [Macrococcus canis]
MATITIRVSEDEKNFLKYMSEFMNLSLTDLIKNYTIEDLEDLFDTHVADKAYIEWSKHKQTVSHNDVMQEFGLL

Nucleotide

• Length: 225 bp

>AT200327 NZ_CP097829:c152115-151831 [Shigella flexneri]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA