Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2030764..2031255 Replicon chromosome
Accession NZ_CP073804
Organism Macrococcus canis strain Epi0100-OL

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag KFV07_RS10705 Protein ID WP_254256056.1
Coordinates 2030764..2031114 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag KFV07_RS10710 Protein ID WP_101495293.1
Coordinates 2031115..2031255 (-) Length 47 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV07_RS10680 (KFV07_10680) 2025872..2028007 - 2136 WP_254256054.1 Tex family protein -
KFV07_RS10685 (KFV07_10685) 2028084..2028851 - 768 WP_133420462.1 RNA polymerase sigma factor SigB -
KFV07_RS10690 (KFV07_10690) 2028856..2029299 - 444 WP_254256055.1 anti-sigma B factor RsbW -
KFV07_RS10695 (KFV07_10695) 2029304..2029627 - 324 WP_086043353.1 anti-sigma factor antagonist -
KFV07_RS10700 (KFV07_10700) 2029708..2030715 - 1008 WP_210152200.1 PP2C family protein-serine/threonine phosphatase -
KFV07_RS10705 (KFV07_10705) 2030764..2031114 - 351 WP_254256056.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFV07_RS10710 (KFV07_10710) 2031115..2031255 - 141 WP_101495293.1 antitoxin MazE Antitoxin
KFV07_RS10715 (KFV07_10715) 2031317..2032459 - 1143 WP_254256057.1 alanine racemase -
KFV07_RS10720 (KFV07_10720) 2032485..2032841 - 357 WP_254256058.1 holo-ACP synthase -
KFV07_RS10725 (KFV07_10725) 2032783..2033304 - 522 WP_211552563.1 PH domain-containing protein -
KFV07_RS10730 (KFV07_10730) 2033288..2034799 - 1512 WP_254256059.1 PH domain-containing protein -
KFV07_RS10735 (KFV07_10735) 2034789..2035265 - 477 WP_138072455.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12732.90 Da        Isoelectric Point: 9.4944

>T200325 WP_254256056.1 NZ_CP073804:c2031114-2030764 [Macrococcus canis]
MKRGDVYLADLSPVKGSEQGGKRPVVIIQNNIGNKHSPTVIVAAITAKINKAKIPTHVEIGKTTYHLEKDSVILLEQIRT
IDKDRLIDHLTVLDKAKMKEVDVALLISLGLSIDSE

Download         Length: 351 bp

>T200325 NZ_CP097829:131187-131585 [Shigella flexneri]
ATGCTGAAGTTTATTCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAAAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 47 a.a.        Molecular weight: 5319.89 Da        Isoelectric Point: 3.8301

>AT200325 WP_101495293.1 NZ_CP073804:c2031255-2031115 [Macrococcus canis]
MSQKLEQMLIEGYQSMAEINLSIASEHYILECEVNDNHQTYTLDGE

Download         Length: 141 bp

>AT200325 NZ_CP097829:130960-131187 [Shigella flexneri]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTAATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCACCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References