Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 2062850..2063341 | Replicon | chromosome |
| Accession | NZ_CP073799 | ||
| Organism | Macrococcus canis strain Epi0089 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | - |
| Locus tag | KFV05_RS10785 | Protein ID | WP_086043355.1 |
| Coordinates | 2062850..2063200 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | - |
| Locus tag | KFV05_RS10790 | Protein ID | WP_101495293.1 |
| Coordinates | 2063201..2063341 (-) | Length | 47 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KFV05_RS10765 (KFV05_10775) | 2060170..2060937 | - | 768 | WP_133420462.1 | RNA polymerase sigma factor SigB | - |
| KFV05_RS10770 (KFV05_10780) | 2060942..2061385 | - | 444 | WP_086043352.1 | anti-sigma B factor RsbW | - |
| KFV05_RS10775 (KFV05_10785) | 2061390..2061713 | - | 324 | WP_086043353.1 | anti-sigma factor antagonist | - |
| KFV05_RS10780 (KFV05_10790) | 2061794..2062801 | - | 1008 | WP_086043354.1 | PP2C family protein-serine/threonine phosphatase | - |
| KFV05_RS10785 (KFV05_10795) | 2062850..2063200 | - | 351 | WP_086043355.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| KFV05_RS10790 (KFV05_10800) | 2063201..2063341 | - | 141 | WP_101495293.1 | antitoxin MazE | Antitoxin |
| KFV05_RS10795 (KFV05_10805) | 2063403..2064545 | - | 1143 | WP_254246221.1 | alanine racemase | - |
| KFV05_RS10800 (KFV05_10810) | 2064571..2064927 | - | 357 | WP_164953884.1 | holo-ACP synthase | - |
| KFV05_RS10805 (KFV05_10815) | 2064869..2065390 | - | 522 | WP_252456021.1 | PH domain-containing protein | - |
| KFV05_RS10810 (KFV05_10820) | 2065374..2066885 | - | 1512 | WP_254246222.1 | PH domain-containing protein | - |
| KFV05_RS10815 (KFV05_10825) | 2066875..2067351 | - | 477 | WP_254246223.1 | PH domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12705.88 Da Isoelectric Point: 9.9423
>T200320 WP_086043355.1 NZ_CP073799:c2063200-2062850 [Macrococcus canis]
MKRGDVYLADLSPVKGSEQGGKRPVVIIQNNIGNKHSPTVIVAAITAKINKAKIPTHVEIGKTTHHLEKDSVILLEQIRT
IDKNRLIDHLTVLDKAKMKEVDVALLISLGLSIDSE
MKRGDVYLADLSPVKGSEQGGKRPVVIIQNNIGNKHSPTVIVAAITAKINKAKIPTHVEIGKTTHHLEKDSVILLEQIRT
IDKNRLIDHLTVLDKAKMKEVDVALLISLGLSIDSE
Download Length: 351 bp
>T200320 NZ_CP097828:4003651-4003806 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA
Antitoxin
Download Length: 47 a.a. Molecular weight: 5319.89 Da Isoelectric Point: 3.8301
>AT200320 WP_101495293.1 NZ_CP073799:c2063341-2063201 [Macrococcus canis]
MSQKLEQMLIEGYQSMAEINLSIASEHYILECEVNDNHQTYTLDGE
MSQKLEQMLIEGYQSMAEINLSIASEHYILECEVNDNHQTYTLDGE
Download Length: 141 bp
>AT200320 NZ_CP097828:c4003639-4003581 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|