200319

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 1404285..1405393 Replicon chromosome
Accession NZ_CP073799
Organism Macrococcus canis strain Epi0089

Toxin (Protein)


Gene name MNTss Uniprot ID R3JIN1
Locus tag KFV05_RS07180 Protein ID WP_000233000.1
Coordinates 1404285..1405154 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag KFV05_RS07185 Protein ID WP_000205227.1
Coordinates 1405169..1405393 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV05_RS07160 (KFV05_07165) 1400556..1401875 - 1320 WP_002338419.1 IS1380-like element ISSsu5 family transposase -
KFV05_RS07165 (KFV05_07170) 1402103..1402630 - 528 WP_002294507.1 phosphoribosyltransferase family protein -
KFV05_RS07170 (KFV05_07175) 1402674..1403537 - 864 WP_002294505.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
KFV05_RS07175 (KFV05_07180) 1403570..1404304 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
KFV05_RS07180 (KFV05_07185) 1404285..1405154 - 870 WP_000233000.1 nucleotidyltransferase domain-containing protein Toxin
KFV05_RS07185 (KFV05_07190) 1405169..1405393 - 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
KFV05_RS07190 (KFV05_07195) 1405536..1407080 - 1545 WP_002390960.1 recombinase family protein -
KFV05_RS07195 (KFV05_07200) 1407100..1407516 - 417 WP_000323438.1 recombinase -
KFV05_RS07200 (KFV05_07205) 1407517..1407966 - 450 WP_224984586.1 DNA recombinase -
KFV05_RS07205 (KFV05_07210) 1408310..1409200 - 891 WP_254245886.1 SMI1/KNR4 family protein -
KFV05_RS07210 (KFV05_07215) 1409267..1410262 - 996 WP_254245887.1 SMI1/KNR4 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn ant(6)-Ia - 1400556..1403537 2981


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32926.57 Da        Isoelectric Point: 4.8781

>T200319 WP_000233000.1 NZ_CP073799:c1405154-1404285 [Macrococcus canis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

>T200319 NZ_CP097828:c3873993-3873886 [Shigella flexneri]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTGCCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT200319 WP_000205227.1 NZ_CP073799:c1405393-1405169 [Macrococcus canis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp

>AT200319 NZ_CP097828:3874040-3874106 [Shigella flexneri]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3JIN1

Antitoxin

Source ID Structure

References