Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1989593..1990084 Replicon chromosome
Accession NZ_CP073798
Organism Macrococcus canis strain KM2258

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag KFV04_RS10335 Protein ID WP_086043355.1
Coordinates 1989593..1989943 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag KFV04_RS10340 Protein ID WP_101495293.1
Coordinates 1989944..1990084 (-) Length 47 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV04_RS10310 (KFV04_10300) 1984701..1986836 - 2136 WP_254254875.1 Tex family protein -
KFV04_RS10315 (KFV04_10305) 1986913..1987680 - 768 WP_133420462.1 RNA polymerase sigma factor SigB -
KFV04_RS10320 (KFV04_10310) 1987685..1988128 - 444 WP_086043352.1 anti-sigma B factor RsbW -
KFV04_RS10325 (KFV04_10315) 1988133..1988456 - 324 WP_086043353.1 anti-sigma factor antagonist -
KFV04_RS10330 (KFV04_10320) 1988537..1989544 - 1008 WP_086043354.1 PP2C family protein-serine/threonine phosphatase -
KFV04_RS10335 (KFV04_10325) 1989593..1989943 - 351 WP_086043355.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFV04_RS10340 (KFV04_10330) 1989944..1990084 - 141 WP_101495293.1 antitoxin MazE Antitoxin
KFV04_RS10345 (KFV04_10335) 1990146..1991288 - 1143 WP_086043356.1 alanine racemase -
KFV04_RS10350 (KFV04_10340) 1991314..1991670 - 357 WP_086043357.1 holo-ACP synthase -
KFV04_RS10355 (KFV04_10345) 1991612..1992133 - 522 WP_236365977.1 PH domain-containing protein -
KFV04_RS10360 (KFV04_10350) 1992117..1993628 - 1512 WP_236365978.1 PH domain-containing protein -
KFV04_RS10365 (KFV04_10355) 1993618..1994094 - 477 WP_254254876.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12705.88 Da        Isoelectric Point: 9.9423

>T200317 WP_086043355.1 NZ_CP073798:c1989943-1989593 [Macrococcus canis]
MKRGDVYLADLSPVKGSEQGGKRPVVIIQNNIGNKHSPTVIVAAITAKINKAKIPTHVEIGKTTHHLEKDSVILLEQIRT
IDKNRLIDHLTVLDKAKMKEVDVALLISLGLSIDSE

Download         Length: 351 bp

>T200317 NZ_CP097828:c3033245-3033027 [Shigella flexneri]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 47 a.a.        Molecular weight: 5319.89 Da        Isoelectric Point: 3.8301

>AT200317 WP_101495293.1 NZ_CP073798:c1990084-1989944 [Macrococcus canis]
MSQKLEQMLIEGYQSMAEINLSIASEHYILECEVNDNHQTYTLDGE

Download         Length: 141 bp

>AT200317 NZ_CP097828:c3033645-3033271 [Shigella flexneri]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References