Detailed information of TA system TA200316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1163043..1163530
• Replicon: chromosome
• Accession: NZ_CP073798
• Organism: Macrococcus canis strain KM2258

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KFV04_RS05770
• Protein ID: WP_236365522.1
• Coordinates: 1163294..1163530 (+)
• Length: 79 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KFV04_RS05765
• Protein ID: WP_236365521.1
• Coordinates: 1163043..1163267 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV04_RS05720 (KFV04_05700)	1158494..1159435	+	942	WP_254254477.1	LCP family protein	-
KFV04_RS05725 (KFV04_05705)	1159473..1159661	-	189	WP_086042483.1	2-hydroxymuconate tautomerase	-
KFV04_RS05730 (KFV04_05710)	1159710..1160714	-	1005	WP_254254478.1	cation diffusion facilitator family transporter	-
KFV04_RS05735 (KFV04_05715)	1160876..1161262	+	387	WP_254254479.1	sporulation protein	-
KFV04_RS05740 (KFV04_05720)	1161337..1161588	+	252	WP_254254480.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KFV04_RS05745 (KFV04_05725)	1161588..1161800	+	213	WP_254254481.1	type II toxin-antitoxin system YoeB family toxin	-
KFV04_RS05750 (KFV04_05730)	1161784..1162209	+	426	WP_254254482.1	hypothetical protein	-
KFV04_RS05755 (KFV04_05735)	1162232..1162504	-	273	WP_254254483.1	Txe/YoeB family addiction module toxin	-
KFV04_RS05760 (KFV04_05740)	1162497..1162754	-	258	WP_086042496.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KFV04_RS05765 (KFV04_05745)	1163043..1163267	+	225	WP_236365521.1	DUF6290 family protein	Antitoxin
KFV04_RS05770 (KFV04_05750)	1163294..1163530	+	237	WP_236365522.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KFV04_RS05775 (KFV04_05755)	1163641..1164009	+	369	WP_254254484.1	GntR family transcriptional regulator	-
KFV04_RS05780 (KFV04_05760)	1164009..1165109	+	1101	WP_254254485.1	DUF1648 domain-containing protein	-
KFV04_RS05785 (KFV04_05765)	1165551..1166813	+	1263	WP_236366941.1	Y-family DNA polymerase	-
KFV04_RS05790 (KFV04_05770)	1166966..1167154	+	189	WP_254254486.1	hypothetical protein	-
KFV04_RS05795 (KFV04_05775)	1167287..1168327	+	1041	WP_254253295.1	M42 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (18-77)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 79 a.a.
• Molecular weight: 9239.79 Da
• Isoelectric Point: 10.7716

>T200316 WP_236365522.1 NZ_CP073798:1163294-1163530 [Macrococcus canis]
VIKQLRKLDAQSSRLLLQWINKNIVHCEDPRSSGKALTGNYSGFWRYRVGQFRIITKIEDDKLIILVISIDHRKNIYR

Nucleotide

• Length: 237 bp

>T200316 NZ_CP097828:c2996394-2996236 [Shigella flexneri]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8745.91 Da
• Isoelectric Point: 4.4193

>AT200316 WP_236365521.1 NZ_CP073798:1163043-1163267 [Macrococcus canis]
MATITIRVSEDEKNFLKYMSEFMNLSLTDLIKNYTIEDLEDLFDTHVADKAYIEWSKHKQTVSHNDVMQEFGLL

Nucleotide

• Length: 225 bp

>AT200316 NZ_CP097828:c2996689-2996378 [Shigella flexneri]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA