Detailed information of TA system TA200311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2189595..2190226
• Replicon: chromosome
• Accession: NZ_CP073797
• Organism: Dactylosporangium matsuzakiense strain NRRL B-16293

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: Dmats_RS09620
• Protein ID: WP_261963473.1
• Coordinates: 2189595..2190020 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: Dmats_RS09625
• Protein ID: WP_261963474.1
• Coordinates: 2190017..2190226 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Dmats_RS09605 (Dmats_09560)	2185901..2187361	+	1461	WP_223102175.1	argininosuccinate synthase	-
Dmats_RS09610 (Dmats_09565)	2187464..2188171	-	708	WP_261963471.1	uracil-DNA glycosylase	-
Dmats_RS09615 (Dmats_09570)	2188182..2189537	-	1356	WP_261963472.1	MFS transporter	-
Dmats_RS09620 (Dmats_09575)	2189595..2190020	-	426	WP_261963473.1	PIN domain nuclease	Toxin
Dmats_RS09625 (Dmats_09580)	2190017..2190226	-	210	WP_261963474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
Dmats_RS09630 (Dmats_09585)	2190288..2190884	-	597	WP_261963475.1	MBL fold metallo-hydrolase	-
Dmats_RS09635 (Dmats_09590)	2190881..2191549	-	669	WP_261963476.1	maleylpyruvate isomerase family mycothiol-dependent enzyme	-
Dmats_RS09640 (Dmats_09595)	2191559..2191993	-	435	WP_261963477.1	hypothetical protein	-
Dmats_RS09645 (Dmats_09600)	2192086..2193120	+	1035	WP_261963478.1	ribosome small subunit-dependent GTPase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (4-34)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15500.81 Da
• Isoelectric Point: 5.5353

>T200311 WP_261963473.1 NZ_CP073797:c2190020-2189595 [Dactylosporangium matsuzakiense]
MTPVRFLVDTSALVRLLRNHQLRVRWEPQITAGVIGICPITELELLHSARSKTDREEWLELLNATFAWVPMPEQIFVRAT
EVQAAMTASGTHRSAGAVDLLLAATAELSGLTLVHYDHDFDEIVKTTGQAAVWLAPAGSIS

Nucleotide

• Length: 426 bp

>T200311 NZ_CP097828:c1636577-1636302 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGAAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7408.41 Da
• Isoelectric Point: 5.0323

>AT200311 WP_261963474.1 NZ_CP073797:c2190226-2190017 [Dactylosporangium matsuzakiense]
MTKVLIDIDDDALAEASALLGTKTKKDTVNTALRSTADQLRRAKALTRLAELGEAGAFDELLDKSAYRG

Nucleotide

• Length: 210 bp

>AT200311 NZ_CP097828:c1636305-1635946 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA