Detailed information of TA system TA200310

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-RelB
• Location: 584142..584668
• Replicon: chromosome
• Accession: NZ_CP073797
• Organism: Dactylosporangium matsuzakiense strain NRRL B-16293

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: Dmats_RS02645
• Protein ID: WP_261962522.1
• Coordinates: 584142..584396 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: Dmats_RS02650
• Protein ID: WP_261962523.1
• Coordinates: 584393..584668 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Dmats_RS02615 (Dmats_02595)	580295..580744	-	450	WP_223100824.1	GatB/YqeY domain-containing protein	-
Dmats_RS02620 (Dmats_02600)	580810..581703	+	894	WP_261962518.1	metallophosphoesterase	-
Dmats_RS02625 (Dmats_02605)	581700..582854	+	1155	WP_261962519.1	prolyl oligopeptidase family serine peptidase	-
Dmats_RS02635 (Dmats_02615)	583377..583640	-	264	WP_261962520.1	hypothetical protein	-
Dmats_RS02640 (Dmats_02620)	583637..583927	-	291	WP_261962521.1	Uma2 family endonuclease	-
Dmats_RS02645 (Dmats_02625)	584142..584396	-	255	WP_261962522.1	Txe/YoeB family addiction module toxin	Toxin
Dmats_RS02650 (Dmats_02630)	584393..584668	-	276	WP_261962523.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
Dmats_RS02655 (Dmats_02635)	584804..586402	+	1599	WP_261961890.1	IS1182 family transposase	-
Dmats_RS02660 (Dmats_02640)	586672..586977	-	306	WP_261962524.1	hypothetical protein	-
Dmats_RS02665 (Dmats_02645)	587228..587956	-	729	WP_261962525.1	GntR family transcriptional regulator	-
Dmats_RS02670 (Dmats_02650)	588040..588576	-	537	WP_261962526.1	hypothetical protein	-
Dmats_RS02675 (Dmats_02655)	588573..589109	-	537	WP_261962527.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10271.56 Da
• Isoelectric Point: 9.5948

>T200310 WP_261962522.1 NZ_CP073797:c584396-584142 [Dactylosporangium matsuzakiense]
VKVQFASQGWDDYLSWRQNRQMLRRVNLLIEDVRRNGHEGIGKPEQLRGNWSGFWSRRIDQEHRLVYRIVDDVVQIAQCR
YHYE

Nucleotide

• Length: 255 bp

>T200310 NZ_CP097828:c1275867-1275553 [Shigella flexneri]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9945.06 Da
• Isoelectric Point: 4.3016

>AT200310 WP_261962523.1 NZ_CP073797:c584668-584393 [Dactylosporangium matsuzakiense]
MRTMSYSEVRQNLAKVLDQVVDDAEEVVVTRSGHEAAVIISLREYESLKETAYLMASPANARRLNDAIEELRTGGGEVHD
LIDPDGNTAAA

Nucleotide

• Length: 276 bp

>AT200310 NZ_CP097828:c1275556-1275140 [Shigella flexneri]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA