Detailed information of TA system TA200309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 6154..6797
• Replicon: plasmid unnamed_2
• Accession: NZ_CP073793
• Organism: Klebsiella pneumoniae isolate KP_NORM_BLD_2010_71621

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: KFO61_RS26955
• Protein ID: WP_001044770.1
• Coordinates: 6381..6797 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: KFO61_RS26950
• Protein ID: WP_001261282.1
• Coordinates: 6154..6384 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFO61_RS26915 (KFO61_26915)	1479..2105	+	627	WP_016946792.1	ParA family plasmid-partitioning AAA ATPase	-
KFO61_RS27680	2102..2404	+	303	WP_004197636.1	hypothetical protein	-
KFO61_RS26925 (KFO61_26925)	2860..3654	-	795	WP_032437528.1	site-specific integrase	-
KFO61_RS26930 (KFO61_26930)	3889..4871	-	983	Protein_4	hypothetical protein	-
KFO61_RS26935 (KFO61_26935)	5041..5346	-	306	WP_032437525.1	type II toxin-antitoxin system toxin CcdB	-
KFO61_RS26940 (KFO61_26940)	5348..5566	-	219	WP_032437523.1	type II toxin-antitoxin system antitoxin CcdA	-
KFO61_RS26945 (KFO61_26945)	5736..6197	-	462	Protein_7	hypothetical protein	-
KFO61_RS26950 (KFO61_26950)	6154..6384	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KFO61_RS26955 (KFO61_26955)	6381..6797	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
KFO61_RS26960 (KFO61_26960)	6871..8433	+	1563	WP_004206609.1	AAA family ATPase	-
KFO61_RS26965 (KFO61_26965)	8418..9440	+	1023	WP_000361404.1	helicase UvrD	-
KFO61_RS26970 (KFO61_26970)	9984..10892	+	909	WP_032411229.1	HNH endonuclease	-
KFO61_RS26975 (KFO61_26975)	11078..11428	-	351	WP_065801210.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	dfrA14 / qnrS1 / blaSHV-12 / aac(3)-IId / aph(3'')-Ib / aph(6)-Id / tet(A)	-	1..82998	82998

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T200309 WP_001044770.1 NZ_CP073793:6381-6797 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T200309 NZ_CP097828:c1075145-1074738 [Shigella flexneri]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT200309 WP_001261282.1 NZ_CP073793:6154-6384 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT200309 NZ_CP097828:c1075392-1075126 [Shigella flexneri]
ATGGACATTAACAACAAAGCCCGAATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3