Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5041..5566 Replicon plasmid unnamed_2
Accession NZ_CP073793
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2010_71621

Toxin (Protein)


Gene name ccdB Uniprot ID A0A8H9ZYZ5
Locus tag KFO61_RS26935 Protein ID WP_032437525.1
Coordinates 5041..5346 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A853HFQ6
Locus tag KFO61_RS26940 Protein ID WP_032437523.1
Coordinates 5348..5566 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO61_RS26915 (KFO61_26915) 1479..2105 + 627 WP_016946792.1 ParA family plasmid-partitioning AAA ATPase -
KFO61_RS27680 2102..2404 + 303 WP_004197636.1 hypothetical protein -
KFO61_RS26925 (KFO61_26925) 2860..3654 - 795 WP_032437528.1 site-specific integrase -
KFO61_RS26930 (KFO61_26930) 3889..4871 - 983 Protein_4 hypothetical protein -
KFO61_RS26935 (KFO61_26935) 5041..5346 - 306 WP_032437525.1 type II toxin-antitoxin system toxin CcdB Toxin
KFO61_RS26940 (KFO61_26940) 5348..5566 - 219 WP_032437523.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KFO61_RS26945 (KFO61_26945) 5736..6197 - 462 Protein_7 hypothetical protein -
KFO61_RS26950 (KFO61_26950) 6154..6384 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin -
KFO61_RS26955 (KFO61_26955) 6381..6797 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
KFO61_RS26960 (KFO61_26960) 6871..8433 + 1563 WP_004206609.1 AAA family ATPase -
KFO61_RS26965 (KFO61_26965) 8418..9440 + 1023 WP_000361404.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid dfrA14 / qnrS1 / blaSHV-12 / aac(3)-IId / aph(3'')-Ib / aph(6)-Id / tet(A) - 1..82998 82998


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11587.24 Da        Isoelectric Point: 6.4650

>T200308 WP_032437525.1 NZ_CP073793:c5346-5041 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLSSARLLSDKVSRDLYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T200308 NZ_CP097828:c143512-143357 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8420.39 Da        Isoelectric Point: 4.7558

>AT200308 WP_032437523.1 NZ_CP073793:c5566-5348 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISRLVNTAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFAEENRDW

Download         Length: 219 bp

>AT200308 NZ_CP097828:143524-143582 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A853HFQ6

References