Detailed information of TA system TA200307

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 76336..76913
• Replicon: plasmid unnamed_1
• Accession: NZ_CP073792
• Organism: Klebsiella pneumoniae isolate KP_NORM_BLD_2010_71621

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A7G3NNX6
• Locus tag: KFO61_RS26425
• Protein ID: WP_059065587.1
• Coordinates: 76336..76668 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A7G3NP82
• Locus tag: KFO61_RS26430
• Protein ID: WP_032454911.1
• Coordinates: 76668..76913 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFO61_RS26405 (KFO61_26405)	72536..72985	+	450	WP_105910642.1	AfaD family invasin	-
KFO61_RS26410 (KFO61_26410)	73103..74290	+	1188	Protein_78	ISL3-like element ISCfr12 family transposase	-
KFO61_RS26415 (KFO61_26415)	74333..75742	+	1410	Protein_79	group II intron reverse transcriptase/maturase	-
KFO61_RS26420 (KFO61_26420)	76179..76271	-	93	Protein_80	IS3 family transposase	-
KFO61_RS26425 (KFO61_26425)	76336..76668	-	333	WP_059065587.1	endoribonuclease MazF	Toxin
KFO61_RS26430 (KFO61_26430)	76668..76913	-	246	WP_032454911.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KFO61_RS26435 (KFO61_26435)	77618..77953	+	336	WP_170933330.1	TonB family protein	-
KFO61_RS26440 (KFO61_26440)	78002..78694	+	693	WP_094313752.1	Exc2 family lipoprotein	-
KFO61_RS26445 (KFO61_26445)	78891..79148	-	258	WP_060655703.1	cloacin immunity family protein	-
KFO61_RS26450 (KFO61_26450)	79158..79352	-	195	Protein_86	colicin E3/pyocin S6 family cytotoxin	-
KFO61_RS26455 (KFO61_26455)	79354..79734	-	381	Protein_87	transposase family protein	-
KFO61_RS26460 (KFO61_26460)	79854..79967	-	114	Protein_88	helix-turn-helix domain-containing protein	-
KFO61_RS26465 (KFO61_26465)	80054..80308	+	255	Protein_89	IS1 family transposase	-
KFO61_RS26470 (KFO61_26470)	80443..81018	+	576	WP_094313438.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iutA / iucD / iucC / iucB / iucA / cseA	1..155175	155175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11991.87 Da
• Isoelectric Point: 7.7494

>T200307 WP_059065587.1 NZ_CP073792:c76668-76336 [Klebsiella pneumoniae]
MVSRFVPDAGDLIWINFDPVEGHEQGGHRPAVVLSPFAYNNKTGLLLCVPCTTKVKGYPFEVELPGERDGVALADQITCV
DWRARKVEKKGKVATGELSEIRAKAKALIG

Nucleotide

• Length: 333 bp

>T200307 NZ_CP097810:2671659-2671835 [Clostridium sp. MD294]
ATGAGGGACAAAGATTTATTAAAACTACTCAAAAAAAACGGTTGGGTAGTAAAGCGAGTAAGAGGAAGCCATCATGTTAT
GCAAAAAGGTGACAAAATACAAATCGTTGCAGTACACAACAAAGATGTTCCTATAGGCTTGCTGGAAAAAATATTAAAGG
AGACAGGGCTAAAATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8881.21 Da
• Isoelectric Point: 5.7996

>AT200307 WP_032454911.1 NZ_CP073792:c76913-76668 [Klebsiella pneumoniae]
MSQATFKKWGNQAGIRFPAAVMKAAALNVDDTVDIEVQEGRIVLIPTKEKTYSLDALLSGITEENMHNKADFGKPTGKEM
L

Nucleotide

• Length: 246 bp

>AT200307 NZ_CP097810:2671871-2672254 [Clostridium sp. MD294]
ATGTTATTTATTTATCCTGCTATTTTTCATAAAGAAAATAATTCCTATTGGGTAGAATTTCCAGACCTTGAAGGTTGCCA
AACATATGGTGATACTTTAAATGAAACTATGGAATATGCTCAAGAGGCATTAGCTGCCTATTTATTGACATTGTTAGAGC
AAAATTTAACTTTAGCACAACCATCTGATATTTCTTCTATATCACATAGTTCAGATAGTTTTGCTACACTTGTGACTTGT
GACATAAATCAATATAAAGATACAAAAGCAGTGAAAAAAACATTGACTATTCCTGCTTGGCTAAATGAAAGAGCATTAGC
AATGAATATCAATTTTTCTCAAGTACTACAAGAAGCTCTTTTGACAAAAATACAAACAAAATAA

Structures

Toxin

• AlphaFold DB: A0A7G3NNX6

Antitoxin

• AlphaFold DB: A0A7G3NP82