Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 89519..90255 Replicon plasmid unnamed_1
Accession NZ_CP073789
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2015_115359

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KFO62_RS25960 Protein ID WP_003026803.1
Coordinates 89773..90255 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KFO62_RS25955 Protein ID WP_003026799.1
Coordinates 89519..89785 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO62_RS25910 (KFO62_25895) 85581..85943 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
KFO62_RS25915 (KFO62_25900) 85993..86343 - 351 WP_117119089.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KFO62_RS25920 (KFO62_25905) 86701..86970 + 270 WP_004152102.1 hypothetical protein -
KFO62_RS25925 (KFO62_25910) 86958..87533 + 576 WP_004152103.1 hypothetical protein -
KFO62_RS25930 (KFO62_25915) 87564..88058 + 495 WP_004152104.1 DNA-binding protein -
KFO62_RS25935 (KFO62_25920) 88102..88470 + 369 WP_004152105.1 hypothetical protein -
KFO62_RS25940 (KFO62_25925) 88504..88707 + 204 WP_004152106.1 HHA domain-containing protein -
KFO62_RS25945 (KFO62_25930) 88756..89013 + 258 WP_004152107.1 hypothetical protein -
KFO62_RS25950 (KFO62_25935) 89089..89343 + 255 WP_004152108.1 hypothetical protein -
KFO62_RS25955 (KFO62_25940) 89519..89785 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KFO62_RS25960 (KFO62_25945) 89773..90255 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KFO62_RS25965 (KFO62_25950) 90434..91837 + 1404 WP_004113090.1 ISNCY family transposase -
KFO62_RS25970 (KFO62_25955) 91868..92785 - 918 WP_158003452.1 S-4TM family putative pore-forming effector -
KFO62_RS25975 (KFO62_25960) 92789..93787 - 999 WP_001610306.1 hypothetical protein -
KFO62_RS25980 (KFO62_25965) 93991..95156 - 1166 Protein_99 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iutA / iucD / iucC / iucB / iucA 1..225831 225831


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T200285 WP_003026803.1 NZ_CP073789:89773-90255 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T200285 NZ_CP097799:c2300006-2299680 [Pasteurella multocida]
ATGGACAGAGTCCGATTTAATCTTACGGAAGATAGCTTCCAAGACAAGTATCTGTATGAGTATTTTTTATATGGAAGTAA
GCATAAAAATATTCCAAGTGATATAGAAAATACGCTAGCGCATAAATTAGATATGTTAGATAGCGCAAATACTTTGAGTG
ATTTACGAAGTCCACCTGGTAATAAATTGGAAAAATTGGAGCCTAAAACGAGTGGGCTATATTCTATCAGAGTGAATGTG
AAGTATCGTTTAATATTTAAATATAAAGAAAGTAATTCTCCAAAAATAACAGAGTTGAGATTAGATAAACATCAATATAG
ATTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT200285 WP_003026799.1 NZ_CP073789:89519-89785 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT200285 NZ_CP097799:c2299621-2299295 [Pasteurella multocida]
ATGAGTAGAGTACAAAGAAAGCCATCAACAGTGGGTGATATTTTATTAGATGAATATTTAGTTCCACTAAATTTGAAAAT
TGCAGATTTAGCTACAATGTTAGATGTTCATCGTAATACAGCAAGTGCACTCGTTAATAATAATACTAAATTGTCTTTAG
AAATGGCACTGAAACTAGCAAAACTATTTAATACAAGTCCTGAGTTTTGGTTAAATTTACAAATGAAATTAGACTTATGG
GAAATTGAAAACAATGCTAGGTTTCAAGAAAGTTTAAAGAAAATTTCGTCTATTGATCAGCATAATCTGTTAGAAAAAAT
CGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References