Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5200504..5201129 Replicon chromosome
Accession NZ_CP073788
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2015_115359

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KFO62_RS25145 Protein ID WP_002882817.1
Coordinates 5200504..5200887 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KFO62_RS25150 Protein ID WP_004150355.1
Coordinates 5200887..5201129 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO62_RS25130 (5197870) 5197870..5198772 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KFO62_RS25135 (5198769) 5198769..5199404 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KFO62_RS25140 (5199401) 5199401..5200330 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KFO62_RS25145 (5200504) 5200504..5200887 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KFO62_RS25150 (5200887) 5200887..5201129 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KFO62_RS25155 (5201334) 5201334..5202251 + 918 WP_009484979.1 alpha/beta hydrolase -
KFO62_RS25160 (5202265) 5202265..5203206 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KFO62_RS25165 (5203251) 5203251..5203688 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KFO62_RS25170 (5203685) 5203685..5204545 - 861 WP_004146232.1 virulence factor BrkB family protein -
KFO62_RS25175 (5204539) 5204539..5205138 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T200284 WP_002882817.1 NZ_CP073788:c5200887-5200504 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T200284 NZ_CP097799:c1139905-1139723 [Pasteurella multocida]
GTGAACTCACACGACTTAATCAAGGAACTGACAGCAATTGGCTGTACTGAATTGAGATGTAAAGGGTCGCACCATATATG
GTATTCACCTAAAACAGGAAAGACGTTTCCTGTTCCGCATCCCAAGAAAGATTTACCAATAGGTACCGTCAGATCCATAA
AGAAATCGGCAGGGCTTTTATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT200284 WP_004150355.1 NZ_CP073788:c5201129-5200887 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT200284 NZ_CP097799:c1139694-1139260 [Pasteurella multocida]
ATGTTATTTACTATTGGTGTTGAAACGCCAAAAAATGAAAATGAAGCGTTTGGCTTATGTGTGCCTGCTCTTTTTAATGA
AACATATTCTTGTTTTAGTGCAGCTGATACAGTTGAAGAAATTATTCCAACAGTGACTGATGCTATCTATACAATTTTGG
AGATAATGGTCGAAGATAACTTCGACATTTCACAAATAAAAGATTTGGGTTTCATGCATTACAAACAACAAAAGGATTTT
GAATTTTGCGATAGCTGGTTGTTAATTGATGTTGATATCACAGCTTATTTCGGTAAGCGTCAACGTGTGAATGTTGTGCT
ACCACAATATTTAATTGACAGAATAGATCAGCGTGTAGCAAATAACCCAACTTACAAAGATCGCAGCCATTTCTTAACTA
TTGCTTCACAACGAGAGCTATCATCATCTTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References