Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3985671..3986290 Replicon chromosome
Accession NZ_CP073788
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2015_115359

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KFO62_RS19300 Protein ID WP_002892050.1
Coordinates 3986072..3986290 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KFO62_RS19295 Protein ID WP_002892066.1
Coordinates 3985671..3986045 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO62_RS19285 (3980823) 3980823..3982016 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFO62_RS19290 (3982039) 3982039..3985185 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KFO62_RS19295 (3985671) 3985671..3986045 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KFO62_RS19300 (3986072) 3986072..3986290 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KFO62_RS19305 (3986449) 3986449..3987015 + 567 WP_002892030.1 maltose O-acetyltransferase -
KFO62_RS19310 (3986987) 3986987..3987127 - 141 WP_004147370.1 hypothetical protein -
KFO62_RS19315 (3987148) 3987148..3987618 + 471 WP_002892026.1 YlaC family protein -
KFO62_RS19320 (3987593) 3987593..3989043 - 1451 Protein_3791 PLP-dependent aminotransferase family protein -
KFO62_RS19325 (3989144) 3989144..3989842 + 699 WP_002892021.1 GNAT family protein -
KFO62_RS19330 (3989839) 3989839..3989979 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KFO62_RS19335 (3989979) 3989979..3990242 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T200280 WP_002892050.1 NZ_CP073788:3986072-3986290 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T200280 NZ_CP073788:3986072-3986290 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT200280 WP_002892066.1 NZ_CP073788:3985671-3986045 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT200280 NZ_CP073788:3985671-3986045 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References