Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17449..18092 Replicon plasmid unnamed_2
Accession NZ_CP073785
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2014_96855

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KFO63_RS26275 Protein ID WP_001044770.1
Coordinates 17676..18092 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KFO63_RS26270 Protein ID WP_001261282.1
Coordinates 17449..17679 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO63_RS26235 (KFO63_26245) 12647..13120 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
KFO63_RS26240 (KFO63_26250) 14334..15116 - 783 WP_004152340.1 site-specific integrase -
KFO63_RS26245 (KFO63_26255) 15116..15448 - 333 WP_004152339.1 hypothetical protein -
KFO63_RS26250 (KFO63_26260) 15455..15853 - 399 WP_004171440.1 hypothetical protein -
KFO63_RS26255 (KFO63_26265) 15879..16208 - 330 WP_004152337.1 hypothetical protein -
KFO63_RS26260 (KFO63_26270) 16236..16544 - 309 WP_004152336.1 hypothetical protein -
KFO63_RS26265 (KFO63_26275) 17031..17492 - 462 WP_072093212.1 hypothetical protein -
KFO63_RS26270 (KFO63_26280) 17449..17679 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KFO63_RS26275 (KFO63_26285) 17676..18092 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KFO63_RS26280 (KFO63_26290) 18166..18855 + 690 Protein_18 AAA family ATPase -
KFO63_RS26285 (KFO63_26295) 18910..19607 + 698 WP_223174630.1 IS1 family transposase -
KFO63_RS26290 (KFO63_26300) 19829..20386 - 558 WP_001217881.1 recombinase family protein -
KFO63_RS26295 (KFO63_26305) 20550..20768 + 219 WP_001143771.1 DUF4158 domain-containing protein -
KFO63_RS26300 (KFO63_26310) 20950..22110 + 1161 Protein_22 IS1380-like element ISEcp1 family transposase -
KFO63_RS26305 (KFO63_26315) 22175..22872 + 698 WP_223174630.1 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / sul1 / cmlA1 / ant(3'')-Ia / ere(A) / aac(3)-IIa - 1..115132 115132
- flank IS/Tn - - 11259..12527 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T200268 WP_001044770.1 NZ_CP073785:17676-18092 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T200268 NZ_CP097793:c777974-777648 [Pasteurella multocida]
ATGGACAGAGTCCGATTTAATCTTACGGAAGATAGCTTCCAAGACAAGTATCTGTATGAGTATTTTTTATATGGAAGTAA
GCATAAAAATATTCCAAGTGATATAGAAAATACGCTAGCGCATAAATTAGATATGTTAGATAGCGCAAATACTTTGAATG
ATTTGCGAAGTCCACCAGGTAATAAATTGGAAAAATTGGAGCCTAAAACGAGTGGGCTATATTCTATCAGAGTGAATGTG
AAATATCGTTTAATATTTAAATATAAAGAAAGTAATTTTCCAAAAATAACAGAGTTGAGATTAGATAAACATCAATATAG
ATTATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT200268 WP_001261282.1 NZ_CP073785:17449-17679 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT200268 NZ_CP097793:c777533-777207 [Pasteurella multocida]
ATGAGTAGAGTACAAAGAAAGCCATCAACAGTGGGTGATATTTTATTAGATGAATATTTAGTTCCACTAAATTTGAAAAT
TGCAGATTTAGCTACAATGTTAGATGTTCATCGTAATACAGCAAGTGCACTCGTTAATAATAATACTAAATTGTCTTTAG
AAATGGCACTGAAACTAGCAAAACTATTTAATACAAGTCCTGAGTTTTGGTTAAATTTACAAATGAAATTAGACTTATGG
GAAATTGAAAACAATGCTAGGTTTCAAGAAAGTTTAAAGAAAATTTTGTCTATTGATCAGCATAACCTGTTAGAAAAAAT
CGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References