Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 112324..113060 Replicon plasmid unnamed_1
Accession NZ_CP073784
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2014_96855

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag KFO63_RS25640 Protein ID WP_004098919.1
Coordinates 112324..112806 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag KFO63_RS25645 Protein ID WP_004213599.1
Coordinates 112794..113060 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO63_RS25620 (KFO63_25630) 107678..109024 + 1347 WP_011154555.1 dihydroorotase -
KFO63_RS25625 (KFO63_25635) 109088..110110 + 1023 WP_004214536.1 porphobilinogen synthase -
KFO63_RS25630 (KFO63_25640) 110285..110680 + 396 Protein_127 IS3 family transposase -
KFO63_RS25635 (KFO63_25645) 110676..111903 - 1228 Protein_128 IS3 family transposase -
KFO63_RS25640 (KFO63_25650) 112324..112806 - 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
KFO63_RS25645 (KFO63_25655) 112794..113060 - 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
KFO63_RS25650 (KFO63_25660) 113255..113485 - 231 WP_004213598.1 hypothetical protein -
KFO63_RS25655 (KFO63_25665) 113499..113702 - 204 WP_004213596.1 HHA domain-containing protein -
KFO63_RS25660 (KFO63_25670) 113763..114257 - 495 WP_004213594.1 hypothetical protein -
KFO63_RS25665 (KFO63_25675) 114299..117268 - 2970 WP_004213592.1 Tn3 family transposase -
KFO63_RS25670 (KFO63_25680) 117271..117828 - 558 WP_004213590.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB 1..218832 218832
- inside IScluster/Tn - - 110676..117828 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T200267 WP_004098919.1 NZ_CP073784:c112806-112324 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T200267 NZ_CP097791:c2246242-2245916 [Pasteurella multocida]
ATGGACAGAGTCCGATTTAATCTTACGGAAGATAGCTTCCAAGACAAGTATCTGTATGAGTATTTTTTATATGGAAGTAA
GCATAAAAATATTCCAAGTGATATAGAAAATACGCTAGCGCATAAATTAGATATGTTAGATAGCGCAAATACTTTGAGTG
ATTTACGAAGTCCACCTGGTAATAAATTGGAAAAATTGGAGCCTAAAACGACTGGGCTATATTCTATCAGAGTGAATGTG
AAGTATCGTTTAATATTTAAATATAAAGAAAGTAATTCTCCAAAAATAACAGAGTTGAGATTAGATAAACATCAATATAG
ATTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT200267 WP_004213599.1 NZ_CP073784:c113060-112794 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT200267 NZ_CP097791:c2245829-2245503 [Pasteurella multocida]
ATGAGTAGAGTACAAAGAAAGCCATCAACAGTGGGTGATATTTTATTAGATGAATATTTAGTTCCACTAAATTTGAAAAT
TGCAGATTTAGCTACAATGTTAGATGTTCATCGTAATACAGCAAGTGCACTCGTTAATAATAATACTAAATTGTCTTTAG
AAATGGCACTGAAACTAGCAAAACTATTTAATACAAGTCCTGAGTTTTGGTTAAATTTACAAATGAAATTAGACTTATGG
GAAATTGAAAACAATGCTAGGTTTCAAGAAAGTTTAAAGAAAATTTCGTCTATTGATCAGCATAATCTGTTAGAAAAAAT
CGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References