Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 74656..75566 Replicon plasmid unnamed_1
Accession NZ_CP073784
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2014_96855

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag KFO63_RS25450 Protein ID WP_004026354.1
Coordinates 75096..75566 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag KFO63_RS25445 Protein ID WP_004026357.1
Coordinates 74656..75099 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO63_RS25415 (KFO63_25425) 69674..70195 + 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -
KFO63_RS25420 (KFO63_25430) 70192..71145 + 954 Protein_83 fec operon regulator FecR -
KFO63_RS25425 (KFO63_25435) 71232..73358 + 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
KFO63_RS25430 (KFO63_25440) 73433..73552 + 120 Protein_85 Fe3+-citrate ABC transporter substrate-binding protein -
KFO63_RS25435 (KFO63_25445) 73733..73954 + 222 WP_004213643.1 DUF2188 domain-containing protein -
KFO63_RS25440 (KFO63_25450) 74052..74555 + 504 Protein_87 DUF4113 domain-containing protein -
KFO63_RS25445 (KFO63_25455) 74656..75099 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
KFO63_RS25450 (KFO63_25460) 75096..75566 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
KFO63_RS25455 (KFO63_25465) 76012..76709 + 698 WP_223175001.1 IS1-like element IS1A family transposase -
KFO63_RS25460 (KFO63_25470) 78095..78745 - 651 WP_068893702.1 DUF1173 family protein -
KFO63_RS27590 78778..79044 - 267 WP_223175074.1 DUF1173 family protein -
KFO63_RS25465 (KFO63_25475) 79222..79716 + 495 WP_004212794.1 thermonuclease family protein -
KFO63_RS25470 (KFO63_25480) 80053..80451 + 399 WP_004212796.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB 1..218832 218832
- flank IS/Tn - - 76206..76709 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T200266 WP_004026354.1 NZ_CP073784:75096-75566 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T200266 NZ_CP097784:c1138870-1138124 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
AGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCGCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGAGTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGGACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT200266 WP_004026357.1 NZ_CP073784:74656-75099 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT200266 NZ_CP097784:c1138123-1137953 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References