Detailed information of TA system TA200265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 19836..20506
• Replicon: plasmid unnamed_1
• Accession: NZ_CP073784
• Organism: Klebsiella pneumoniae isolate KP_NORM_BLD_2014_96855

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: KFO63_RS25135
• Protein ID: WP_004213072.1
• Coordinates: 20063..20506 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: KFO63_RS25130
• Protein ID: WP_004213073.1
• Coordinates: 19836..20066 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFO63_RS25105 (KFO63_25115)	16059..16958	-	900	WP_004225022.1	nucleotide-binding protein	-
KFO63_RS25110 (KFO63_25120)	16948..17238	-	291	WP_004213078.1	hypothetical protein	-
KFO63_RS25115 (KFO63_25125)	17590..17796	+	207	WP_004213077.1	hypothetical protein	-
KFO63_RS25120 (KFO63_25130)	17786..18079	-	294	WP_004213076.1	hypothetical protein	-
KFO63_RS25125 (KFO63_25135)	18095..19228	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
KFO63_RS25130 (KFO63_25140)	19836..20066	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KFO63_RS25135 (KFO63_25145)	20063..20506	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
KFO63_RS27570	20655..20906	+	252	WP_186987481.1	hypothetical protein	-
KFO63_RS25145 (KFO63_25155)	20929..21233	-	305	Protein_27	transposase	-
KFO63_RS25150 (KFO63_25160)	21650..22285	+	636	Protein_28	mucoid phenotype regulator RmpA2	-
KFO63_RS25155 (KFO63_25165)	22803..23206	-	404	Protein_29	GAF domain-containing protein	-
KFO63_RS25160 (KFO63_25170)	23297..24217	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
KFO63_RS25165 (KFO63_25175)	24266..24757	-	492	WP_011251327.1	DM13 domain-containing protein	-
KFO63_RS25170 (KFO63_25180)	24820..25095	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	1..218832	218832

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T200265 WP_004213072.1 NZ_CP073784:20063-20506 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T200265 NZ_CP097784:385765-386115 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT200265 WP_004213073.1 NZ_CP073784:19836-20066 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT200265 NZ_CP097784:385478-385759 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620