Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5144089..5144714 Replicon chromosome
Accession NZ_CP073783
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2014_96855

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag KFO63_RS24655 Protein ID WP_019705794.1
Coordinates 5144089..5144472 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KFO63_RS24660 Protein ID WP_004150355.1
Coordinates 5144472..5144714 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO63_RS24640 (KFO63_24650) 5141455..5142357 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KFO63_RS24645 (KFO63_24655) 5142354..5142989 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KFO63_RS24650 (KFO63_24660) 5142986..5143915 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KFO63_RS24655 (KFO63_24665) 5144089..5144472 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
KFO63_RS24660 (KFO63_24670) 5144472..5144714 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KFO63_RS24665 (KFO63_24675) 5144919..5145836 + 918 WP_019705795.1 alpha/beta hydrolase -
KFO63_RS24670 (KFO63_24680) 5145850..5146791 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KFO63_RS24675 (KFO63_24685) 5146836..5147273 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KFO63_RS24680 (KFO63_24690) 5147270..5148130 - 861 WP_069339404.1 virulence factor BrkB family protein -
KFO63_RS24685 (KFO63_24695) 5148124..5148723 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T200264 WP_019705794.1 NZ_CP073783:c5144472-5144089 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T200264 NZ_CP097779:c1068584-1067838 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
AGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCGCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGAGTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGGACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT200264 WP_004150355.1 NZ_CP073783:c5144714-5144472 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT200264 NZ_CP097779:c1067837-1067667 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References