Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4540841..4541651 Replicon chromosome
Accession NZ_CP073783
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2014_96855

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KFO63_RS21865 Protein ID WP_004178461.1
Coordinates 4540841..4541374 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KFO63_RS21870 Protein ID WP_002887278.1
Coordinates 4541385..4541651 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO63_RS21860 (KFO63_21875) 4539672..4540793 + 1122 WP_002887282.1 cupin domain-containing protein -
KFO63_RS21865 (KFO63_21880) 4540841..4541374 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KFO63_RS21870 (KFO63_21885) 4541385..4541651 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KFO63_RS21875 (KFO63_21890) 4541754..4543187 - 1434 WP_019705874.1 Cu(+)/Ag(+) sensor histidine kinase -
KFO63_RS21880 (KFO63_21895) 4543177..4543860 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KFO63_RS21885 (KFO63_21900) 4544032..4545417 + 1386 WP_032409188.1 efflux transporter outer membrane subunit -
KFO63_RS21890 (KFO63_21905) 4545435..4545779 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T200261 WP_004178461.1 NZ_CP073783:c4541374-4540841 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T200261 NZ_CP097775:4477969-4478319 [Paenibacillus polymyxa]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGCCCTACCGTGATCGTAGCAGCCATCACAGCACAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATC
CGGACGATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
ACAGATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT200261 WP_002887278.1 NZ_CP073783:c4541651-4541385 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT200261 NZ_CP097775:4477681-4477962 [Paenibacillus polymyxa]
TTGGCCAATTTGCAGAACACCAAAAGAATAATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGC
TATGGAGAATTCCAACCGCAGTGAATTTATCAGGCAGGCAATGAAGCTTTATGTAGGTGAACGTAAAAAACGTTACATTC
GTGAAGCTATGCAGCGTGGATACATGGAAATGGCTAAAATTAACCTGACCATGGCATCTGAAGCCTTTCACGCGGAGGAA
GATGCAAACAGCACCTTGGGCCGTACAGTTAGCGGGGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References