Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3930037..3930656 Replicon chromosome
Accession NZ_CP073783
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2014_96855

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KFO63_RS18935 Protein ID WP_002892050.1
Coordinates 3930438..3930656 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KFO63_RS18930 Protein ID WP_002892066.1
Coordinates 3930037..3930411 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO63_RS18920 (KFO63_18935) 3925189..3926382 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFO63_RS18925 (KFO63_18940) 3926405..3929551 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KFO63_RS18930 (KFO63_18945) 3930037..3930411 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KFO63_RS18935 (KFO63_18950) 3930438..3930656 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KFO63_RS18940 (KFO63_18955) 3930815..3931381 + 567 WP_002892030.1 maltose O-acetyltransferase -
KFO63_RS18945 (KFO63_18960) 3931353..3931493 - 141 WP_032409038.1 hypothetical protein -
KFO63_RS18950 (KFO63_18965) 3931514..3931984 + 471 WP_002892026.1 YlaC family protein -
KFO63_RS18955 (KFO63_18970) 3931959..3933409 - 1451 Protein_3723 PLP-dependent aminotransferase family protein -
KFO63_RS18960 (KFO63_18975) 3933510..3934208 + 699 WP_019705329.1 GNAT family protein -
KFO63_RS18965 (KFO63_18980) 3934205..3934345 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KFO63_RS18970 (KFO63_18985) 3934345..3934608 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T200260 WP_002892050.1 NZ_CP073783:3930438-3930656 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T200260 NZ_CP097773:4465904-4466254 [Paenibacillus polymyxa]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGCCCTACCGTGATCGTAGCAGCCATCACAGCACAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATC
CGGACGATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
ACAGATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT200260 WP_002892066.1 NZ_CP073783:3930037-3930411 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT200260 NZ_CP097773:4465616-4465897 [Paenibacillus polymyxa]
TTGGCCAATTTGCAGAACACCAAAAGAATAATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGC
TATGGAGAATTCCAACCGCAGTGAATTTATCAGGCAGGCAATGAAGCTTTATGTAGGTGAACGTAAAAAACGTTACATTC
GTGAAGCTATGCAGCGTGGATACATGGAAATGGCTAAAATTAACCTGACCATGGCATCTGAAGCCTTTCACGCGGAGGAA
GATGCAAACAGCACCTTGGGCCGTACAGTTAGCGGGGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References