Detailed information of TA system TA200258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2633739..2635327
• Replicon: chromosome
• Accession: NZ_CP073783
• Organism: Klebsiella pneumoniae isolate KP_NORM_BLD_2014_96855

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KFO63_RS12805
• Protein ID: WP_019705665.1
• Coordinates: 2633739..2635061 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KFO63_RS12810
• Protein ID: WP_004176198.1
• Coordinates: 2635061..2635327 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFO63_RS12790 (KFO63_12780)	2628795..2631296	+	2502	WP_064181310.1	fimbria/pilus outer membrane usher protein	-
KFO63_RS12795 (KFO63_12785)	2631284..2632288	+	1005	WP_004184029.1	fimbrial protein	-
KFO63_RS12800 (KFO63_12790)	2632430..2633722	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
KFO63_RS12805 (KFO63_12795)	2633739..2635061	-	1323	WP_019705665.1	type II toxin-antitoxin system HipA family toxin	Toxin
KFO63_RS12810 (KFO63_12800)	2635061..2635327	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KFO63_RS12815 (KFO63_12805)	2635593..2635919	-	327	WP_019705664.1	hydroxyisourate hydrolase	-
KFO63_RS12820 (KFO63_12810)	2635916..2636416	-	501	WP_019705663.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KFO63_RS12825 (KFO63_12815)	2636394..2637773	-	1380	WP_004179808.1	nucleobase:cation symporter-2 family protein	-
KFO63_RS12830 (KFO63_12820)	2638119..2639273	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
KFO63_RS12835 (KFO63_12825)	2639254..2640177	-	924	WP_019705662.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49155.46 Da
• Isoelectric Point: 7.5358

>T200258 WP_019705665.1 NZ_CP073783:c2635061-2633739 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T200258 NZ_CP097728:c97478-97200 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
TGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT200258 WP_004176198.1 NZ_CP073783:c2635327-2635061 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT200258 NZ_CP097728:c97747-97478 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA