Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 5264366..5265176 Replicon chromosome
Accession NZ_CP073782
Organism Klebsiella pneumoniae strain IRQBAS106c-genome

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KFU55_RS25755 Protein ID WP_004178461.1
Coordinates 5264366..5264899 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KFU55_RS25760 Protein ID WP_002887278.1
Coordinates 5264910..5265176 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU55_RS25735 (5260433) 5260433..5261839 - 1407 Protein_5062 Tn3-like element IS3000 family transposase -
KFU55_RS25740 (5262114) 5262114..5262449 + 336 Protein_5063 IS6-like element IS26 family transposase -
KFU55_RS25745 (5262503) 5262503..5262751 - 249 Protein_5064 class II aldolase/adducin family protein -
KFU55_RS25750 (5263197) 5263197..5264318 + 1122 WP_009309849.1 cupin domain-containing protein -
KFU55_RS25755 (5264366) 5264366..5264899 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KFU55_RS25760 (5264910) 5264910..5265176 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KFU55_RS25765 (5265279) 5265279..5266712 - 1434 WP_023280821.1 Cu(+)/Ag(+) sensor histidine kinase -
KFU55_RS25770 (5266702) 5266702..5267385 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KFU55_RS25775 (5267558) 5267558..5268943 + 1386 WP_087793843.1 efflux transporter outer membrane subunit -
KFU55_RS25780 (5268961) 5268961..5269305 + 345 WP_048266266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T200244 WP_004178461.1 NZ_CP073782:c5264899-5264366 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T200244 NZ_CP097726:2903912-2904406 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT200244 WP_002887278.1 NZ_CP073782:c5265176-5264910 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT200244 NZ_CP097726:2903622-2903915 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References