200241

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4474456..4475081 Replicon chromosome
Accession NZ_CP073782
Organism Klebsiella pneumoniae strain IRQBAS106c-genome

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KFU55_RS21755 Protein ID WP_002882817.1
Coordinates 4474698..4475081 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KFU55_RS21750 Protein ID WP_004150355.1
Coordinates 4474456..4474698 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU55_RS21725 (4470447) 4470447..4471046 + 600 WP_004151865.1 glucose-1-phosphatase -
KFU55_RS21730 (4471040) 4471040..4471900 + 861 WP_004146232.1 virulence factor BrkB family protein -
KFU55_RS21735 (4471897) 4471897..4472334 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KFU55_RS21740 (4472379) 4472379..4473320 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KFU55_RS21745 (4473334) 4473334..4474251 - 918 WP_117260249.1 alpha/beta hydrolase -
KFU55_RS21750 (4474456) 4474456..4474698 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KFU55_RS21755 (4474698) 4474698..4475081 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KFU55_RS21760 (4475255) 4475255..4476184 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KFU55_RS21765 (4476181) 4476181..4476816 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KFU55_RS21770 (4476813) 4476813..4477715 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T200241 WP_002882817.1 NZ_CP073782:4474698-4475081 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T200241 NZ_CP097726:c2604205-2604029 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT200241 WP_004150355.1 NZ_CP073782:4474456-4474698 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT200241 NZ_CP097726:c2603983-2603567 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References