Detailed information of TA system TA200240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4188718..4190306
• Replicon: chromosome
• Accession: NZ_CP073782
• Organism: Klebsiella pneumoniae strain IRQBAS106c-genome

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: J2LTC9
• Locus tag: KFU55_RS20290
• Protein ID: WP_004148372.1
• Coordinates: 4188718..4190040 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KFU55_RS20295
• Protein ID: WP_004176198.1
• Coordinates: 4190040..4190306 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU55_RS20275 (4183774)	4183774..4186275	+	2502	WP_040025883.1	fimbria/pilus outer membrane usher protein	-
KFU55_RS20280 (4186263)	4186263..4187267	+	1005	WP_040025882.1	fimbrial protein	-
KFU55_RS20285 (4187409)	4187409..4188701	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
KFU55_RS20290 (4188718)	4188718..4190040	-	1323	WP_004148372.1	type II toxin-antitoxin system HipA family toxin	Toxin
KFU55_RS20295 (4190040)	4190040..4190306	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KFU55_RS20300 (4190571)	4190571..4190897	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
KFU55_RS20305 (4190894)	4190894..4191394	-	501	WP_020804988.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KFU55_RS20310 (4191372)	4191372..4192751	-	1380	WP_206552300.1	nucleobase:cation symporter-2 family protein	-
KFU55_RS20315 (4193097)	4193097..4194251	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
KFU55_RS20320 (4194232)	4194232..4195155	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49127.45 Da
• Isoelectric Point: 7.5357

>T200240 WP_004148372.1 NZ_CP073782:c4190040-4188718 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSLRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T200240 NZ_CP097726:2554724-2555002 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCATCGACTTGCTGTGATTGATGCGGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT200240 WP_004176198.1 NZ_CP073782:c4190306-4190040 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT200240 NZ_CP097726:2555002-2555286 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: J2LTC9