Detailed information of TA system TA020024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1489716..1490354
• Replicon: chromosome
• Accession: CP009789
• Organism: Escherichia coli K-12 strain ER3413

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: ER3413_4551
• Protein ID: AIZ54218.1
• Coordinates: 1489716..1489892 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ER3413_1470
• Protein ID: AIZ51237.1
• Coordinates: 1489938..1490354 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER3413_1464	1485335..1486510	-	1176	AIZ51233.1	inner membrane protein, predicted transporter, function unknown	-
ER3413_1465	1486602..1487138	+	537	AIZ51234.1	putative DNA-binding transcriptional regulator	-
ER3413_1466	1487211..1489172	+	1962	AIZ51235.1	putative peptidase	-
ER3413_1467	1489264..1489494	-	231	AIZ51236.1	hypothetical protein	-
ER3413_4551	1489716..1489892	+	177	AIZ54218.1	mRNA interferase toxin of the HicAB toxin-antitoxin system	Toxin
ER3413_1470	1489938..1490354	+	417	AIZ51237.1	antitoxin for the HicAB toxin-antitoxin system	Antitoxin
ER3413_1471	1490433..1491839	+	1407	AIZ51238.1	fused predicted DNA-binding transcriptional regulator/predicted amino transferase	-
ER3413_1472	1492084..1493229	+	1146	AIZ51239.1	polyhydroxybutyrate (PHB) synthase, ABC transporter periplasmic binding protein homolog	-
ER3413_1473	1493247..1494260	+	1014	AIZ51240.1	putative spermidine/putrescine transporter subunit	-
ER3413_1474	1494261..1495202	+	942	AIZ51241.1	putative spermidine/putrescine transporter subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T20024 AIZ54218.1 CP009789:1489716-1489892 [Escherichia coli K-12]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T20024 CP009789:1489716-1489892 [Escherichia coli K-12]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT20024 AIZ51237.1 CP009789:1489938-1490354 [Escherichia coli K-12]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT20024 CP009789:1489938-1490354 [Escherichia coli K-12]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA