Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 111820..112559 Replicon plasmid pYUSHP2-2
Accession NZ_CP073773
Organism Enterobacter hormaechei strain SH19PTE2

Toxin (Protein)


Gene name tacT Uniprot ID A0A411GR51
Locus tag KFO54_RS24680 Protein ID WP_032664832.1
Coordinates 111820..112305 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A411GR70
Locus tag KFO54_RS24685 Protein ID WP_032664831.1
Coordinates 112293..112559 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO54_RS24655 (106903) 106903..107544 - 642 WP_045295096.1 TerD family protein -
KFO54_RS24660 (107567) 107567..108205 - 639 WP_045295093.1 VWA domain-containing protein -
KFO54_RS24665 (108530) 108530..109417 + 888 WP_131638989.1 hypothetical protein -
KFO54_RS24670 (109816) 109816..110226 + 411 WP_063402002.1 hypothetical protein -
KFO54_RS24675 (110281) 110281..111569 - 1289 Protein_127 ISNCY family transposase -
KFO54_RS24680 (111820) 111820..112305 - 486 WP_032664832.1 GNAT family N-acetyltransferase Toxin
KFO54_RS24685 (112293) 112293..112559 - 267 WP_032664831.1 DUF1778 domain-containing protein Antitoxin
KFO54_RS24690 (113214) 113214..114227 + 1014 WP_032664830.1 plasmid replication initiator RepA -
KFO54_RS24695 (115120) 115120..115923 + 804 WP_032664829.1 DUF4225 domain-containing protein -
KFO54_RS24700 (115901) 115901..116224 - 324 WP_032664828.1 hypothetical protein -
KFO54_RS24705 (116239) 116239..117009 - 771 WP_032664827.1 TraX family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / dfrA14 / sul2 / floR / tet(A) / blaLAP-2 / qnrS1 - 1..193176 193176
- flank IS/Tn - - 110281..111120 839


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-147)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17593.31 Da        Isoelectric Point: 9.6084

>T200222 WP_032664832.1 NZ_CP073773:c112305-111820 [Enterobacter hormaechei]
VGRVTAPEPLSSCHQLAEFASGETVLDDWLKQRGLKNQALGAARTFVVCKANTKQVAGFYSLATGSVNHVEATGSLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKSFYLHHGFKPSQTQERTLFLKLP
Q

Download         Length: 486 bp

>T200222 NZ_CP097724:2440507-2440785 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10077.50 Da        Isoelectric Point: 4.4733

>AT200222 WP_032664831.1 NZ_CP073773:c112559-112293 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADNVAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT200222 NZ_CP097724:2440785-2441069 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A411GR51

Antitoxin

Source ID Structure
AlphaFold DB A0A411GR70

References