Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 34905..35431 Replicon plasmid pYUSHP2-2
Accession NZ_CP073773
Organism Enterobacter hormaechei strain SH19PTE2

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag KFO54_RS24255 Protein ID WP_000323025.1
Coordinates 35144..35431 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag KFO54_RS24250 Protein ID WP_000534858.1
Coordinates 34905..35144 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO54_RS24235 (30344) 30344..31171 - 828 WP_047365775.1 universal stress protein -
KFO54_RS24240 (31190) 31190..32668 - 1479 WP_047365777.1 SulP family inorganic anion transporter -
KFO54_RS24245 (33201) 33201..34643 - 1443 WP_047365884.1 IS1182 family transposase -
KFO54_RS25640 (34782) 34782..34880 - 99 Protein_41 protein YdfV -
KFO54_RS24250 (34905) 34905..35144 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
KFO54_RS24255 (35144) 35144..35431 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
KFO54_RS24260 (35498) 35498..36532 - 1035 Protein_44 IS481 family transposase -
KFO54_RS24265 (36604) 36604..36762 + 159 WP_013087178.1 type I toxin-antitoxin system Hok family toxin -
KFO54_RS24270 (37638) 37638..37952 + 315 WP_044157807.1 hypothetical protein -
KFO54_RS24275 (38013) 38013..38474 + 462 WP_174402229.1 hypothetical protein -
KFO54_RS24280 (38562) 38562..38762 + 201 WP_013087182.1 hypothetical protein -
KFO54_RS24285 (38803) 38803..39594 + 792 WP_013087183.1 N-6 DNA methylase -
KFO54_RS24290 (39636) 39636..39971 + 336 WP_013087184.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / dfrA14 / sul2 / floR / tet(A) / blaLAP-2 / qnrS1 - 1..193176 193176
- inside IScluster/Tn - - 33201..36532 3331


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T200221 WP_000323025.1 NZ_CP073773:35144-35431 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T200221 NZ_CP097724:2119600-2119702 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT200221 WP_000534858.1 NZ_CP073773:34905-35144 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT200221 NZ_CP097724:c2119740-2119472 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References