Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 92460..94083 Replicon plasmid pYUSHP2-1
Accession NZ_CP073772
Organism Enterobacter hormaechei strain SH19PTE2

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag KFO54_RS23555 Protein ID WP_000088046.1
Coordinates 92460..93785 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag KFO54_RS23560 Protein ID WP_024136327.1
Coordinates 93790..94083 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO54_RS23515 (KFO54_23515) 87481..87837 + 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -
KFO54_RS23520 (KFO54_23520) 88179..88382 - 204 WP_001194555.1 hypothetical protein -
KFO54_RS23525 (KFO54_23525) 88444..89301 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
KFO54_RS23530 (KFO54_23530) 89317..89625 - 309 WP_001224687.1 hypothetical protein -
KFO54_RS23535 (KFO54_23535) 90174..90599 - 426 WP_001043843.1 hypothetical protein -
KFO54_RS23540 (KFO54_23540) 90853..91668 - 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
KFO54_RS23545 (KFO54_23545) 91681..92091 - 411 WP_000985911.1 hypothetical protein -
KFO54_RS23550 (KFO54_23550) 92193..92399 - 207 WP_000134171.1 hypothetical protein -
KFO54_RS23555 (KFO54_23555) 92460..93785 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
KFO54_RS23560 (KFO54_23560) 93790..94083 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
KFO54_RS23565 (KFO54_23565) 94687..95079 - 393 WP_000341066.1 hypothetical protein -
KFO54_RS23570 (KFO54_23570) 95452..95793 + 342 WP_001151575.1 hypothetical protein -
KFO54_RS23575 (KFO54_23575) 95885..96076 + 192 WP_000398480.1 hypothetical protein -
KFO54_RS23580 (KFO54_23580) 96126..96407 + 282 WP_000077926.1 hypothetical protein -
KFO54_RS25705 96748..96879 - 132 WP_000374059.1 hypothetical protein -
KFO54_RS23585 (KFO54_23585) 96970..98211 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-IIc / ere(A) / qacE / sul1 / qnrB4 / blaDHA-1 / dfrA12 / fosA3 / blaCTX-M-14 / qnrS1 / tet(A) - 1..194047 194047


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T200219 WP_000088046.1 NZ_CP073772:c93785-92460 [Enterobacter hormaechei]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T200219 NZ_CP097724:1900649-1900903 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCAGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT200219 WP_024136327.1 NZ_CP073772:c94083-93790 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT200219 NZ_CP097724:1900401-1900652 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAGGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCTGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References