Detailed information of TA system TA200207

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1699183..1699980
• Replicon: chromosome
• Accession: NZ_CP073771
• Organism: Enterobacter hormaechei strain SH19PTE2

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A4Q2R3N2
• Locus tag: KFO54_RS08075
• Protein ID: WP_032670168.1
• Coordinates: 1699459..1699980 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: F5RT92
• Locus tag: KFO54_RS08070
• Protein ID: WP_001303459.1
• Coordinates: 1699183..1699452 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFO54_RS08040 (KFO54_08045)	1694816..1695316	+	501	WP_211989348.1	DedA family protein	-
KFO54_RS08045 (KFO54_08050)	1695409..1696650	+	1242	WP_023315779.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
KFO54_RS08050 (KFO54_08055)	1696683..1696910	-	228	WP_006809324.1	YccJ family protein	-
KFO54_RS08055 (KFO54_08060)	1696929..1697525	-	597	WP_006809323.1	NAD(P)H:quinone oxidoreductase	-
KFO54_RS08060 (KFO54_08065)	1697917..1698087	+	171	WP_001273664.1	general stress protein	-
KFO54_RS08065 (KFO54_08070)	1698204..1699109	+	906	WP_211989346.1	DMT family transporter	-
KFO54_RS08070 (KFO54_08075)	1699183..1699452	+	270	WP_001303459.1	DUF1778 domain-containing protein	Antitoxin
KFO54_RS08075 (KFO54_08080)	1699459..1699980	+	522	WP_032670168.1	GNAT family N-acetyltransferase	Toxin
KFO54_RS08080 (KFO54_08085)	1700039..1701361	-	1323	WP_211989345.1	pyrimidine utilization transport protein G	-
KFO54_RS08085 (KFO54_08090)	1701383..1701877	-	495	WP_003858102.1	pyrimidine utilization flavin reductase protein F	-
KFO54_RS08090 (KFO54_08095)	1701887..1702477	-	591	WP_058652290.1	malonic semialdehyde reductase	-
KFO54_RS08095 (KFO54_08100)	1702487..1703287	-	801	WP_211989343.1	pyrimidine utilization protein D	-
KFO54_RS08100 (KFO54_08105)	1703295..1703681	-	387	WP_003858097.1	pyrimidine utilization protein C	-
KFO54_RS08105 (KFO54_08110)	1703693..1704382	-	690	WP_003858095.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19437.18 Da
• Isoelectric Point: 7.4318

>T200207 WP_032670168.1 NZ_CP073771:1699459-1699980 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLVRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Nucleotide

• Length: 522 bp

>T200207 NZ_CP097721:c4236204-4236031 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10248.70 Da
• Isoelectric Point: 5.8836

>AT200207 WP_001303459.1 NZ_CP073771:1699183-1699452 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT200207 NZ_CP097721:c4236528-4236232 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A4Q2R3N2

Antitoxin

• AlphaFold DB: A0A7D7BCZ2