Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 36994..37541 Replicon plasmid pYUSHP6-3
Accession NZ_CP073770
Organism Escherichia coli strain SH19PTE6

Toxin (Protein)


Gene name parE Uniprot ID -
Locus tag KEH54_RS23305 Protein ID WP_244461891.1
Coordinates 37308..37541 (+) Length 78 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A829FT18
Locus tag KEH54_RS23300 Protein ID WP_000079938.1
Coordinates 36994..37263 (+) Length 90 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEH54_RS23280 (KEH54_23285) 32548..33705 + 1158 WP_000217345.1 alpha-hydroxy acid oxidase -
KEH54_RS23285 (KEH54_23290) 33739..34824 + 1086 WP_001148851.1 iron-containing alcohol dehydrogenase family protein -
KEH54_RS23290 (KEH54_23295) 34914..35225 - 312 WP_024191312.1 hypothetical protein -
KEH54_RS23295 (KEH54_23300) 35279..36259 + 981 WP_071940815.1 IS5-like element ISKpn26 family transposase -
KEH54_RS23300 (KEH54_23305) 36994..37263 + 270 WP_000079938.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
KEH54_RS23305 (KEH54_23310) 37308..37541 + 234 WP_244461891.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KEH54_RS23310 (KEH54_23315) 37599..37730 + 132 Protein_41 IS3 family transposase -
KEH54_RS23320 (KEH54_23325) 38562..38966 + 405 WP_240725492.1 hypothetical protein -
KEH54_RS23325 (KEH54_23330) 39257..40780 + 1524 WP_000921251.1 hypothetical protein -
KEH54_RS23330 (KEH54_23335) 40815..41939 + 1125 WP_032174239.1 DUF4238 domain-containing protein -
KEH54_RS23335 (KEH54_23340) 41939..42229 + 291 WP_000648897.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..64665 64665
- inside IScluster/Tn - - 20487..49099 28612


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-76)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 78 a.a.        Molecular weight: 8940.20 Da        Isoelectric Point: 6.7231

>T200201 WP_244461891.1 NZ_CP073770:37308-37541 [Escherichia coli]
IREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGARELVIHPHFVLVYEVDSQWEKVYILRVLHTTQKWP

Download         Length: 234 bp

>T200201 NZ_CP097721:3414787-3415005 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10251.64 Da        Isoelectric Point: 10.1734

>AT200201 WP_000079938.1 NZ_CP073770:36994-37263 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLKRGDVLPHDMVESSAAAWRD
EMSRKVADK

Download         Length: 270 bp

>AT200201 NZ_CP097721:3414387-3414761 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A829FT18

References