Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3428163..3428781 Replicon chromosome
Accession NZ_CP073768
Organism Escherichia coli strain SH19PTE6

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KEH54_RS16515 Protein ID WP_001291435.1
Coordinates 3428563..3428781 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KEH54_RS16510 Protein ID WP_000344800.1
Coordinates 3428163..3428537 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEH54_RS16500 (3423252) 3423252..3424445 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KEH54_RS16505 (3424468) 3424468..3427617 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KEH54_RS16510 (3428163) 3428163..3428537 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KEH54_RS16515 (3428563) 3428563..3428781 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KEH54_RS16520 (3428953) 3428953..3429504 + 552 WP_000102564.1 maltose O-acetyltransferase -
KEH54_RS16525 (3429620) 3429620..3430090 + 471 WP_000136192.1 YlaC family protein -
KEH54_RS16530 (3430254) 3430254..3431804 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KEH54_RS16535 (3431846) 3431846..3432153 - 308 Protein_3277 DUF1428 family protein -
KEH54_RS16545 (3432532) 3432532..3432843 + 312 WP_000409911.1 MGMT family protein -
KEH54_RS16550 (3432874) 3432874..3433446 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T200194 WP_001291435.1 NZ_CP073768:3428563-3428781 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T200194 NZ_CP097721:1838027-1838281 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT200194 WP_000344800.1 NZ_CP073768:3428163-3428537 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT200194 NZ_CP097721:1837779-1838030 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References