Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09812-MazE
Location 164543..165171 Replicon plasmid pHY1
Accession NZ_CP073765
Organism Methylovirgula sp. HY1

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag MHY1_RS17005 Protein ID WP_219323874.1
Coordinates 164809..165171 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag MHY1_RS17000 Protein ID WP_219323872.1
Coordinates 164543..164809 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MHY1_RS16985 (MHY1_p00165) 161285..162430 - 1146 WP_219323867.1 RHE_PE00001 family protein -
MHY1_RS17720 (MHY1_p00167) 162784..163041 + 258 WP_255565196.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
MHY1_RS16995 (MHY1_p00168) 163071..164243 + 1173 WP_219323870.1 site-specific integrase -
MHY1_RS17000 (MHY1_p00169) 164543..164809 + 267 WP_219323872.1 PbsX family transcriptional regulator Antitoxin
MHY1_RS17005 (MHY1_p00170) 164809..165171 + 363 WP_219323874.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MHY1_RS17010 (MHY1_p00172) 165562..166767 + 1206 WP_219323876.1 plasmid partitioning protein RepA -
MHY1_RS17015 (MHY1_p00173) 166770..167789 + 1020 WP_219323878.1 plasmid partitioning protein RepB -
MHY1_RS17020 (MHY1_p00174) 168122..169351 + 1230 WP_219323880.1 plasmid replication protein RepC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - gmd / ureG 1..278037 278037


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13028.92 Da        Isoelectric Point: 6.4617

>T200171 WP_219323874.1 NZ_CP073765:164809-165171 [Methylovirgula sp. HY1]
MVRSEVPNRGDVYWIDPNPIAGREMKDRHRFVVITPRQINALGVSMTVPITTGGAFTRDAGLSVSISGHETTGVVVCNQV
RSFDIVARVRAGSASFIEMLDKATMDEIIARVVSAIDPES

Download         Length: 363 bp

>T200171 NZ_CP097719:2568227-2569543 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGATCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAACAGCTCACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTGCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGTGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGCAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACCGTCGGTTAAGCCGAGAATGGTATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9522.21 Da        Isoelectric Point: 9.9248

>AT200171 WP_219323872.1 NZ_CP073765:164543-164809 [Methylovirgula sp. HY1]
MAVTSKIRKQGGAAVMTIPPALLKLMHVDVGAQMTLSVQDGKLMAQPVGPTRRRYTLAELLEGSDVMKRLNAEVAWAREG
EPVGHEIV

Download         Length: 267 bp

>AT200171 NZ_CP097719:2567961-2568227 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TGACGACCTTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References