Detailed information of TA system TA200166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2735329..2735897
• Replicon: chromosome
• Accession: NZ_CP073764
• Organism: Methylovirgula sp. HY1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MHY1_RS12815
• Protein ID: WP_219320159.1
• Coordinates: 2735329..2735676 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MHY1_RS12820
• Protein ID: WP_219320160.1
• Coordinates: 2735673..2735897 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MHY1_RS12795 (MHY1_02618)	2730723..2731250	-	528	WP_255564902.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
MHY1_RS17660	2731401..2731952	+	552	Protein_2519	helix-turn-helix domain-containing protein	-
MHY1_RS12800	2731968..2732924	+	957	WP_255565179.1	AlkA N-terminal domain-containing protein	-
MHY1_RS12805 (MHY1_02620)	2733033..2734202	+	1170	WP_219320157.1	radical SAM family heme chaperone HemW	-
MHY1_RS12810 (MHY1_02621)	2734302..2735303	+	1002	WP_219320158.1	adenosine kinase	-
MHY1_RS12815 (MHY1_02622)	2735329..2735676	-	348	WP_219320159.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MHY1_RS12820 (MHY1_02623)	2735673..2735897	-	225	WP_219320160.1	antitoxin MazE family protein	Antitoxin
MHY1_RS12825 (MHY1_02624)	2736003..2736962	-	960	WP_219320161.1	type I pantothenate kinase	-
MHY1_RS12830 (MHY1_02625)	2737117..2737437	-	321	WP_219320162.1	phosphoribosyl-ATP diphosphatase	-
MHY1_RS12835 (MHY1_02626)	2737463..2738245	-	783	WP_219320163.1	imidazole glycerol phosphate synthase subunit HisF	-
MHY1_RS12840 (MHY1_02627)	2738238..2738993	-	756	WP_219320164.1	1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase	-
MHY1_RS12845 (MHY1_02628)	2738981..2739631	-	651	WP_219320165.1	imidazole glycerol phosphate synthase subunit HisH	-
MHY1_RS12850 (MHY1_02629)	2739628..2740110	-	483	WP_219320166.1	DUF2628 domain-containing protein	-
MHY1_RS12855 (MHY1_02630)	2740200..2740787	-	588	WP_219320167.1	imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-104)

Antitoxin

• Antitoxin MazE-like (11-72)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12598.52 Da
• Isoelectric Point: 7.7933

>T200166 WP_219320159.1 NZ_CP073764:c2735676-2735329 [Methylovirgula sp. HY1]
MRRGEIWTVAGGKDYVGKPRPAVIIQDNSFDATDSITICAFTTDETDAPLFRLPVGGTDRNGLNSTCRLMVDKITTVPKS
KIGTFIGQLDEEDILRLNQAVLVFLGLAMSPKARR

Nucleotide

• Length: 348 bp

>T200166 NZ_CP097719:1519152-1519550 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8353.33 Da
• Isoelectric Point: 11.6533

>AT200166 WP_219320160.1 NZ_CP073764:c2735897-2735673 [Methylovirgula sp. HY1]
MVSVNKSNSSRDKVRTHRERLRAQGLRPIQIWVPDMRAPAFRSEAHRQSLAVAQSAHAAEDQSFIDAITDRNNG

Nucleotide

• Length: 225 bp

>AT200166 NZ_CP097719:1518925-1519152 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA