Detailed information of TA system TA200165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2550582..2551233
• Replicon: chromosome
• Accession: NZ_CP073764
• Organism: Methylovirgula sp. HY1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MHY1_RS11950
• Protein ID: WP_219319998.1
• Coordinates: 2550582..2550923 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MHY1_RS11955
• Protein ID: WP_219319999.1
• Coordinates: 2550958..2551233 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MHY1_RS11940 (MHY1_02444)	2546962..2549271	-	2310	WP_219323586.1	NADP-dependent malic enzyme	-
MHY1_RS11945 (MHY1_02445)	2549429..2550448	-	1020	WP_219319997.1	ketol-acid reductoisomerase	-
MHY1_RS11950 (MHY1_02446)	2550582..2550923	-	342	WP_219319998.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MHY1_RS11955 (MHY1_02447)	2550958..2551233	-	276	WP_219319999.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MHY1_RS11960 (MHY1_02448)	2551294..2551845	-	552	WP_219320000.1	acetolactate synthase small subunit	-
MHY1_RS11965 (MHY1_02449)	2551869..2553629	-	1761	WP_219320001.1	acetolactate synthase 3 large subunit	-
MHY1_RS11970 (MHY1_02451)	2554622..2555332	+	711	WP_219320002.1	spermidine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-104)

Antitoxin

• Antitoxin PemI-like (1-65)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12254.11 Da
• Isoelectric Point: 5.0320

>T200165 WP_219319998.1 NZ_CP073764:c2550923-2550582 [Methylovirgula sp. HY1]
VVGAGELVWVDFDPAFGHEQSGRRPALVVSDGGYNQVSSFILVCPITRNPKPWPFKISLSDMFVVDGQVLVDQIKAIDKR
CVVTPALGKVDDEVLSQIRGLLASLLGFSDANR

Nucleotide

• Length: 342 bp

>T200165 NZ_CP097719:1071892-1072299 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10255.80 Da
• Isoelectric Point: 6.3674

>AT200165 WP_219319999.1 NZ_CP073764:c2551233-2550958 [Methylovirgula sp. HY1]
MRVQISKWGNSTALRLPKALVDELGLRLGQEVELVIEGREARLRPLNAIPRYRIEDLVAEMGKLGRNNEPALEDWSAIEA
PWPNDAPKQGK

Nucleotide

• Length: 276 bp

>AT200165 NZ_CP097719:1071645-1071911 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA