Detailed information of TA system TA200163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 26020..26504
• Replicon: chromosome
• Accession: NZ_CP073764
• Organism: Methylovirgula sp. HY1

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: MHY1_RS00135
• Protein ID: WP_219320734.1
• Coordinates: 26020..26289 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: MHY1_RS00140
• Protein ID: WP_219320735.1
• Coordinates: 26286..26504 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MHY1_RS00105 (MHY1_00022)	21628..22020	-	393	WP_219320729.1	response regulator	-
MHY1_RS00110 (MHY1_00023)	22274..22462	+	189	WP_219320730.1	hypothetical protein	-
MHY1_RS00115 (MHY1_00024)	22589..23551	+	963	WP_219323094.1	bifunctional riboflavin kinase/FAD synthetase	-
MHY1_RS00120 (MHY1_00025)	23833..24123	+	291	WP_219320731.1	GIY-YIG nuclease family protein	-
MHY1_RS00125 (MHY1_00026)	24224..25078	-	855	WP_219320732.1	glutamate racemase	-
MHY1_RS00130 (MHY1_00027)	25190..26023	-	834	WP_219320733.1	RNA methyltransferase	-
MHY1_RS00135 (MHY1_00028)	26020..26289	-	270	WP_219320734.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MHY1_RS00140 (MHY1_00029)	26286..26504	-	219	WP_219320735.1	DUF6290 family protein	Antitoxin
MHY1_RS00145 (MHY1_00030)	26946..28157	+	1212	WP_219320736.1	NADP-dependent isocitrate dehydrogenase	-
MHY1_RS00150 (MHY1_00031)	28487..28852	+	366	WP_219320737.1	RNA polymerase-binding protein DksA	-
MHY1_RS00155 (MHY1_00032)	29196..29915	+	720	WP_219320738.1	sulfite exporter TauE/SafE family protein	-
MHY1_RS00160 (MHY1_00033)	30107..31426	+	1320	WP_219320739.1	pitrilysin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Protein of unknown function DUF6290 (3-65)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10349.03 Da
• Isoelectric Point: 10.6033

>T200163 WP_219320734.1 NZ_CP073764:c26289-26020 [Methylovirgula sp. HY1]
MTWQIEFDAAALDDLRKLGSAGRVHVLRFLQERIAPADDPRRLGKALSGDKPGFWRYRVGDYRIIARIEDQRLMVLIIAI
GHRSEIYRK

Nucleotide

• Length: 270 bp

>T200163 NZ_CP097719:c639472-639008 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8325.59 Da
• Isoelectric Point: 4.5230

>AT200163 WP_219320735.1 NZ_CP073764:c26504-26286 [Methylovirgula sp. HY1]
MLALRLPPDIEKRLDELARKTGRTKSYYARKAIIEMIEDLNDLELAKARLAENDERIPLEQILDEFAAELNA

Nucleotide

• Length: 219 bp

>AT200163 NZ_CP097719:c639807-639472 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA