Detailed information of TA system TA200162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3229572..3230171
• Replicon: chromosome
• Accession: NZ_CP073754
• Organism: Methylomonas paludis strain S2AM

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KEF85_RS14725
• Protein ID: WP_215581866.1
• Coordinates: 3229875..3230171 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KEF85_RS14720
• Protein ID: WP_215581864.1
• Coordinates: 3229572..3229871 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEF85_RS14685 (KEF85_14690)	3224648..3225055	-	408	WP_215581854.1	DUF2750 domain-containing protein	-
KEF85_RS14690 (KEF85_14695)	3225248..3225670	-	423	WP_215585186.1	DUF86 domain-containing protein	-
KEF85_RS14695 (KEF85_14700)	3225660..3226076	-	417	WP_246534967.1	nucleotidyltransferase domain-containing protein	-
KEF85_RS14700 (KEF85_14705)	3226515..3227186	+	672	WP_215581856.1	hypothetical protein	-
KEF85_RS14705 (KEF85_14710)	3227545..3228474	-	930	WP_246534968.1	2OG-Fe(II) oxygenase	-
KEF85_RS14710 (KEF85_14715)	3228630..3228950	-	321	WP_215581858.1	hypothetical protein	-
KEF85_RS14715 (KEF85_14720)	3228966..3229208	-	243	WP_215581860.1	UPF0175 family protein	-
KEF85_RS14720 (KEF85_14725)	3229572..3229871	-	300	WP_215581864.1	putative addiction module antidote protein	Antitoxin
KEF85_RS14725 (KEF85_14730)	3229875..3230171	-	297	WP_215581866.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KEF85_RS14730 (KEF85_14735)	3230412..3230849	-	438	WP_246534969.1	GNAT family N-acetyltransferase	-
KEF85_RS14735 (KEF85_14740)	3230918..3231199	-	282	WP_215581869.1	DUF1778 domain-containing protein	-
KEF85_RS14740 (KEF85_14745)	3232641..3233648	+	1008	WP_215581870.1	integrase domain-containing protein	-
KEF85_RS14745 (KEF85_14750)	3233632..3234246	+	615	WP_215581872.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3229572..3256639	27067

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (10-96)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10975.83 Da
• Isoelectric Point: 9.9963

>T200162 WP_215581866.1 NZ_CP073754:c3230171-3229875 [Methylomonas paludis]
MYIIRTLPQFDKWLSGIKDGMTKRRLVARIKKAQLGNLGDIKSVGEGVFEMREHFGAGYRMYYLQHGAVMIIMLGGGDKS
SQADDIAKAKQLANLLED

Nucleotide

• Length: 297 bp

>T200162 NZ_CP097719:331481-331858 [Escherichia coli]
ATGACCATTCAGTTTATCTCAGCGGAGGAGATAATTTATTTCCACGATAAATTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTATGAGTATGAAGGCGTTTCTGATCTTT
GGTTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATCTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCATCATACTTGCTGCAAACCCAGAGTTTGTAGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACGCGGTACCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10606.21 Da
• Isoelectric Point: 5.6079

>AT200162 WP_215581864.1 NZ_CP073754:c3229871-3229572 [Methylomonas paludis]
MTEKISVASLPEFDASHYLDSEHAIAEYLTVIIEDGDSALLAAALGDIARARGMADIAKASGLTREALYKALRPNAHPRF
DTIAKVCKALGVKLQVEPI

Nucleotide

• Length: 300 bp

>AT200162 NZ_CP097719:331263-331484 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGGCAGAACCTTGCATTAGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCAGCTGTGATTATCAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CAGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTAAGGAGTTAGCGGATAAATGA