Detailed information of TA system TA200161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 2720007..2720518
• Replicon: chromosome
• Accession: NZ_CP073754
• Organism: Methylomonas paludis strain S2AM

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KEF85_RS12150
• Protein ID: WP_215580931.1
• Coordinates: 2720159..2720518 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KEF85_RS12145
• Protein ID: WP_215580929.1
• Coordinates: 2720007..2720162 (+)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEF85_RS12115 (KEF85_12120)	2715324..2716404	-	1081	Protein_2388	IS3 family transposase	-
KEF85_RS12120 (KEF85_12125)	2716554..2717045	-	492	WP_215580919.1	DUF3368 domain-containing protein	-
KEF85_RS12125 (KEF85_12130)	2717052..2717396	-	345	WP_215580921.1	UPF0175 family protein	-
KEF85_RS12130 (KEF85_12135)	2717514..2718131	-	618	WP_215580923.1	DUF3780 domain-containing protein	-
KEF85_RS12135 (KEF85_12140)	2718160..2718453	-	294	WP_215580925.1	hypothetical protein	-
KEF85_RS12140 (KEF85_12145)	2718746..2719885	-	1140	WP_215580927.1	IS4 family transposase	-
KEF85_RS12145 (KEF85_12150)	2720007..2720162	+	156	WP_215580929.1	DUF433 domain-containing protein	Antitoxin
KEF85_RS12150 (KEF85_12155)	2720159..2720518	+	360	WP_215580931.1	DUF5615 family PIN-like protein	Toxin
KEF85_RS12155 (KEF85_12160)	2720559..2721362	-	804	WP_215580933.1	TOBE domain-containing protein	-
KEF85_RS12160 (KEF85_12165)	2721564..2721992	+	429	WP_215580935.1	TOBE domain-containing protein	-
KEF85_RS12165 (KEF85_12170)	2722330..2722740	+	411	WP_215580937.1	nucleotidyltransferase substrate binding protein	-
KEF85_RS12170 (KEF85_12175)	2722743..2723048	+	306	WP_215580939.1	nucleotidyltransferase domain-containing protein	-
KEF85_RS12175 (KEF85_12180)	2723232..2723963	-	732	WP_215580941.1	carbonic anhydrase	-
KEF85_RS12180 (KEF85_12185)	2724135..2724719	+	585	WP_215580943.1	cytochrome b/b6 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (1-77)

Antitoxin

• Protein of unknown function DUF433 (1-40)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13749.73 Da
• Isoelectric Point: 4.6533

>T200161 WP_215580931.1 NZ_CP073754:2720159-2720518 [Methylomonas paludis]
MIFWIDAQLPPRLANWLIQTFNEAYALRDLNLRDAEDEQIFHTARRPGIVIISKDSDFVEMVLRLGSPPQLLWVTCGNVT
NTRLENLLLKVFPRAQELLSQGESIVELTDLDSNQYKNH

Nucleotide

• Length: 360 bp

>T200161 NZ_CP097719:303987-304262 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTT
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5730.70 Da
• Isoelectric Point: 4.2667

>AT200161 WP_215580929.1 NZ_CP073754:2720007-2720162 [Methylomonas paludis]
MRIRVSDILQMLGEGVSSDEILLDFPVLEIQDIQACLLYAARRANLERLAA

Nucleotide

• Length: 156 bp

>AT200161 NZ_CP097719:304259-304618 [Escherichia coli]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTACTAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA