Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 2324463..2325124 Replicon chromosome
Accession NZ_CP073754
Organism Methylomonas paludis strain S2AM

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag KEF85_RS10475 Protein ID WP_215580268.1
Coordinates 2324705..2325124 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag KEF85_RS10470 Protein ID WP_215580266.1
Coordinates 2324463..2324708 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEF85_RS10445 (KEF85_10450) 2320650..2321405 + 756 WP_246534864.1 substrate-binding domain-containing protein -
KEF85_RS10450 (KEF85_10455) 2321485..2322060 + 576 WP_215580260.1 c-type cytochrome -
KEF85_RS10455 (KEF85_10460) 2322130..2322204 + 75 WP_215585123.1 pyrroloquinoline quinone precursor peptide PqqA -
KEF85_RS10460 (KEF85_10465) 2322608..2323375 - 768 WP_215580262.1 SDR family NAD(P)-dependent oxidoreductase -
KEF85_RS10465 (KEF85_10470) 2323912..2324061 + 150 WP_215580264.1 type II toxin-antitoxin system ParD family antitoxin -
KEF85_RS10470 (KEF85_10475) 2324463..2324708 + 246 WP_215580266.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KEF85_RS10475 (KEF85_10480) 2324705..2325124 + 420 WP_215580268.1 type II toxin-antitoxin system VapC family toxin Toxin
KEF85_RS16945 2325169..2325297 - 129 WP_281413632.1 hypothetical protein -
KEF85_RS10480 (KEF85_10485) 2325781..2326101 + 321 WP_215580271.1 type II toxin-antitoxin system PrlF family antitoxin -
KEF85_RS10485 (KEF85_10490) 2326101..2326553 + 453 WP_215580273.1 type II toxin-antitoxin system YhaV family toxin -
KEF85_RS10490 (KEF85_10495) 2326744..2327346 - 603 WP_215580275.1 group II intron maturase-specific domain-containing protein -
KEF85_RS10495 (KEF85_10500) 2327527..2327706 - 180 WP_215580277.1 hypothetical protein -
KEF85_RS10500 (KEF85_10505) 2327733..2327909 - 177 WP_215580279.1 hypothetical protein -
KEF85_RS10505 (KEF85_10510) 2327909..2329078 - 1170 WP_215580281.1 putative DNA binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-128)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-61)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15690.13 Da        Isoelectric Point: 6.4525

>T200160 WP_215580268.1 NZ_CP073754:2324705-2325124 [Methylomonas paludis]
MNYLLDTCVLSELIKPQPNQNVATWIEKIQECHLYISALTMGEIQKGISRLENSKKKQSLQIWLEQDLAARFSGRILSVD
AEISLEWGNMQGQAIRSGTTLPVIDSLIAATAKCHNLTLVTRNLEDFKNLPINLNNPWL

Download         Length: 420 bp

>T200160 NZ_CP097719:c44937-44560 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9185.37 Da        Isoelectric Point: 5.6864

>AT200160 WP_215580266.1 NZ_CP073754:2324463-2324708 [Methylomonas paludis]
MHWQLQEAKNKLSQVIHEAQTTGPQTITVRGKETAVVISAADYQKLTDKKDSLLKFFQESPLTDVELDLTRSKESGRDIE
L

Download         Length: 246 bp

>AT200160 NZ_CP097719:c45395-45027 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCATGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCGGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCTAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACCCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References