Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/upstrm_HI1419-dnstrm_HI1420
Location 1737431..1738028 Replicon chromosome
Accession NZ_CP073754
Organism Methylomonas paludis strain S2AM

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag KEF85_RS07905 Protein ID WP_215584760.1
Coordinates 1737732..1738028 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag KEF85_RS07900 Protein ID WP_215584758.1
Coordinates 1737431..1737730 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEF85_RS07880 (KEF85_07885) 1732553..1733692 + 1140 WP_215584752.1 FMNH2-dependent alkanesulfonate monooxygenase -
KEF85_RS07885 (KEF85_07890) 1733696..1734898 + 1203 WP_215584754.1 acyl-CoA dehydrogenase family protein -
KEF85_RS07890 (KEF85_07895) 1734917..1735768 + 852 WP_215584756.1 amidohydrolase family protein -
KEF85_RS07895 (KEF85_07900) 1736363..1736734 - 372 WP_246535078.1 helix-turn-helix domain-containing protein -
KEF85_RS07900 (KEF85_07905) 1737431..1737730 - 300 WP_215584758.1 putative addiction module antidote protein Antitoxin
KEF85_RS07905 (KEF85_07910) 1737732..1738028 - 297 WP_215584760.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KEF85_RS07910 (KEF85_07915) 1738381..1738929 + 549 WP_215584762.1 FMN reductase -
KEF85_RS07915 (KEF85_07920) 1738982..1740070 + 1089 WP_215584764.1 dimethyl sulfone monooxygenase SfnG -
KEF85_RS07920 (KEF85_07925) 1740097..1741239 + 1143 WP_215584766.1 AMP-binding protein -
KEF85_RS07925 (KEF85_07930) 1741236..1742006 + 771 WP_215585086.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-97)

Antitoxin

Uncharacterized protein HI1420

(12-98)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11075.81 Da        Isoelectric Point: 9.7308

>T200159 WP_215584760.1 NZ_CP073754:c1738028-1737732 [Methylomonas paludis]
MTIIRTLPQFDIWLSSIKDNMTRIRLARRLVRAQQGQFGDVKPVGDGVFEMREHFGPGWRMYYVQHGDVVIVMLGGGDKA
TQAADIAKAKRLADTLED

Download         Length: 297 bp

>T200159 NZ_CP097718:30370-30618 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACAGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10640.35 Da        Isoelectric Point: 5.8505

>AT200159 WP_215584758.1 NZ_CP073754:c1737730-1737431 [Methylomonas paludis]
MTKKISVASLPEFDISSLLDNDAAISEYLSQVLDDGDSEELLRALGYIAKAKGMSLIAKESGLGRESLYKALSPGAKPRF
DTIMKVIKALGVKLHAETV

Download         Length: 300 bp

>AT200159 NZ_CP097718:30615-30962 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAATTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References