Detailed information of TA system TA200158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 1655014..1655647
• Replicon: chromosome
• Accession: NZ_CP073754
• Organism: Methylomonas paludis strain S2AM

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KEF85_RS07560
• Protein ID: WP_215584636.1
• Coordinates: 1655234..1655647 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KEF85_RS07555
• Protein ID: WP_215584634.1
• Coordinates: 1655014..1655247 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEF85_RS07540 (KEF85_07545)	1650357..1652399	-	2043	WP_215584630.1	hypothetical protein	-
KEF85_RS07545 (KEF85_07550)	1652637..1654001	-	1365	WP_215580104.1	IS1380 family transposase	-
KEF85_RS07550 (KEF85_07555)	1654500..1654754	+	255	WP_215584632.1	hypothetical protein	-
KEF85_RS07555 (KEF85_07560)	1655014..1655247	+	234	WP_215584634.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KEF85_RS07560 (KEF85_07565)	1655234..1655647	+	414	WP_215584636.1	PIN domain-containing protein	Toxin
KEF85_RS07565 (KEF85_07570)	1655888..1656256	-	369	WP_246535073.1	hypothetical protein	-
KEF85_RS07570 (KEF85_07575)	1656307..1657758	-	1452	WP_215584638.1	sodium:proton antiporter NhaD	-
KEF85_RS07575 (KEF85_07580)	1657976..1658185	+	210	WP_215584640.1	DUF1289 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1652637..1654001	1364

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-116)

Antitoxin

• SpoVT-AbrB domain (5-44)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15360.58 Da
• Isoelectric Point: 4.2870

>T200158 WP_215584636.1 NZ_CP073754:1655234-1655647 [Methylomonas paludis]
MKNDKCFFDTNVLLYMLSGDGEQADHVEAIVSRDGIISVQVLNEFASVASRKLGMTYPEIREYLATIRAVCDVRPVTVET
HLLGLDMAERYGFSFYDSLIVSSALLSDCLTLYSEDMQHGQTVESRLVIVNPFLVEL

Nucleotide

• Length: 414 bp

>T200158 NZ_CP097718:28404-28655 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGTAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 9043.50 Da
• Isoelectric Point: 8.6388

>AT200158 WP_215584634.1 NZ_CP073754:1655014-1655247 [Methylomonas paludis]
MQVAKWGNSLAVRLPAAVVEVLQLKEGDDIEIHIVDERVFELKKKPTHLELFARLRKYRGRLPEGFKFDRLEANEER

Nucleotide

• Length: 234 bp

>AT200158 NZ_CP097718:28642-28869 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA