Detailed information of TA system TA200155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii(toxin)
• Location: 1087677..1088995
• Replicon: chromosome
• Accession: NZ_CP073754
• Organism: Methylomonas paludis strain S2AM

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: KEF85_RS05000
• Protein ID: WP_215583609.1
• Coordinates: 1088078..1088995 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: KEF85_RS16795
• Protein ID: WP_246535043.1
• Coordinates: 1087677..1088081 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEF85_RS04975 (KEF85_04980)	1083889..1084038	-	150	WP_215583604.1	hypothetical protein	-
KEF85_RS04980 (KEF85_04985)	1084447..1085229	+	783	WP_215583605.1	TIGR04255 family protein	-
KEF85_RS04985 (KEF85_04990)	1085239..1085778	+	540	WP_215583606.1	hypothetical protein	-
KEF85_RS04990 (KEF85_04995)	1086099..1086458	+	360	WP_215583607.1	hypothetical protein	-
KEF85_RS16795	1087677..1088081	+	405	WP_246535043.1	hypothetical protein	Antitoxin
KEF85_RS05000 (KEF85_05005)	1088078..1088995	+	918	WP_215583609.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
KEF85_RS05005 (KEF85_05010)	1089503..1091158	-	1656	WP_215579402.1	IS66 family transposase	-
KEF85_RS05010 (KEF85_05015)	1091204..1091557	-	354	WP_215579399.1	IS66 family insertion sequence element accessory protein TnpB	-
KEF85_RS05015 (KEF85_05020)	1091566..1091898	-	333	WP_215579397.1	hypothetical protein	-
KEF85_RS05020 (KEF85_05025)	1091937..1092443	+	507	WP_281413708.1	site-specific integrase	-
KEF85_RS05030 (KEF85_05035)	1092695..1093750	-	1056	WP_215583611.1	UDP-glucose 4-epimerase GalE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1082461..1083825	1364

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-269)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34580.68 Da
• Isoelectric Point: 8.1471

>T200155 WP_215583609.1 NZ_CP073754:1088078-1088995 [Methylomonas paludis]
VSKANVAASVRSRLLNHAREKKQDFNLVLTRYTIERLLYRISISKHADQFLLKGALLFDLWFDIPHRPTRDADFLGFGSA
ELPNIERVFKEVCMIDTPDGVAFQSNTVRAVEIRKESHYAGVRVTMLGVIDGARCHVQVDIGFGDAVTPGPENAEYPVIL
RDFPSPKLRVYPRYTVVAEKFEALSSLGIANSRMKDYFDLWVLACHTEFDGEILCQAVRATFDRRKTTLSEQVPFGLTQA
FAEDQQKQVQWQAFLRKNKLEALTLDEALSKLIVFMMPVVHAANTNNAFQFRWQAGGPWSPVNLS

Nucleotide

• Length: 918 bp

>T200155 NZ_CP097717:c108490-108074 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 15167.74 Da
• Isoelectric Point: 9.2298

>AT200155 WP_246535043.1 NZ_CP073754:1087677-1088081 [Methylomonas paludis]
VARKHPQAIVCLLSALRFHDITTQSPFEVWLGIPNKARAPRMDYPPLRIVRISGAALIEGVEEHRVDGVPVRVTNIARTV
ADCFKYRNKIGLEVALEALQESVREKGVTMDDLWRFATLCRVANVMRPYMESLP

Nucleotide

• Length: 405 bp

>AT200155 NZ_CP097717:c108717-108487 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA