Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 500457..501091 Replicon chromosome
Accession NZ_CP073754
Organism Methylomonas paludis strain S2AM

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag KEF85_RS02410 Protein ID WP_215583147.1
Coordinates 500457..500855 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag KEF85_RS02415 Protein ID WP_215583148.1
Coordinates 500858..501091 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEF85_RS02350 (KEF85_02355) 495555..495977 + 423 WP_215583140.1 putative toxin-antitoxin system toxin component, PIN family -
KEF85_RS02355 (KEF85_02360) 496465..496617 - 153 Protein_466 IS5/IS1182 family transposase -
KEF85_RS02360 (KEF85_02365) 496784..497110 - 327 Protein_467 transposase domain-containing protein -
KEF85_RS02365 (KEF85_02370) 497098..497328 - 231 WP_215579471.1 hypothetical protein -
KEF85_RS02370 (KEF85_02375) 497374..497727 - 354 WP_215579475.1 IS66 family insertion sequence element accessory protein TnpB -
KEF85_RS02375 (KEF85_02380) 497736..498065 - 330 WP_215579477.1 hypothetical protein -
KEF85_RS02380 (KEF85_02385) 498286..498492 - 207 Protein_471 transposase domain-containing protein -
KEF85_RS02385 (KEF85_02390) 498524..498736 + 213 Protein_472 transposase domain-containing protein -
KEF85_RS02390 (KEF85_02395) 498823..499092 - 270 WP_215583143.1 DUF2442 domain-containing protein -
KEF85_RS02395 (KEF85_02400) 499052..499291 - 240 WP_215583144.1 DUF4160 domain-containing protein -
KEF85_RS02400 (KEF85_02405) 499826..500059 + 234 WP_215583145.1 DUF433 domain-containing protein -
KEF85_RS02405 (KEF85_02410) 500056..500388 + 333 WP_215583146.1 DUF5615 family PIN-like protein -
KEF85_RS02410 (KEF85_02415) 500457..500855 - 399 WP_215583147.1 type II toxin-antitoxin system VapC family toxin Toxin
KEF85_RS02415 (KEF85_02420) 500858..501091 - 234 WP_215583148.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KEF85_RS02420 (KEF85_02425) 501391..503064 - 1674 WP_215583149.1 formate--tetrahydrofolate ligase -
KEF85_RS02425 (KEF85_02430) 503118..504374 - 1257 WP_215583150.1 serine hydroxymethyltransferase -
KEF85_RS02430 (KEF85_02435) 504443..505837 - 1395 WP_215583151.1 DUF4147 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(7-55)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14791.13 Da        Isoelectric Point: 8.9461

>T200154 WP_215583147.1 NZ_CP073754:c500855-500457 [Methylomonas paludis]
MLRFLLDTNIVIYVIKQRPIEVLATFNKHHNRMAISIITLAELVHGAEKSQFPAKNLSTIEDFCSRLQVLPYTESAAYHY
GTIRAALELAGQAIGVNNLHIAAHARSQGLVLVSNNLREFNRVPGLLTENWV

Download         Length: 399 bp

>T200154 NZ_CP097717:c103627-103211 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8727.76 Da        Isoelectric Point: 4.7063

>AT200154 WP_215583148.1 NZ_CP073754:c501091-500858 [Methylomonas paludis]
MTTSTVFTNNRSQAVRLPADMRFPESVKKVEVRLVGNERIIAPVESAWDSFFIAGPTVSDDFMTERASQQQSERESF

Download         Length: 234 bp

>AT200154 NZ_CP097717:c103854-103624 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References