Detailed information of TA system TA200153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 494127..494680
• Replicon: chromosome
• Accession: NZ_CP073754
• Organism: Methylomonas paludis strain S2AM

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: KEF85_RS02340
• Protein ID: WP_215584965.1
• Coordinates: 494390..494680 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: KEF85_RS02335
• Protein ID: WP_215583138.1
• Coordinates: 494127..494387 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEF85_RS02310 (KEF85_02315)	490330..490878	+	549	WP_215583134.1	response regulator transcription factor	-
KEF85_RS02315 (KEF85_02320)	490930..491793	+	864	WP_215583135.1	YicC/YloC family endoribonuclease	-
KEF85_RS02320 (KEF85_02325)	491892..492443	+	552	WP_215583136.1	transposase	-
KEF85_RS02325 (KEF85_02330)	492665..492958	+	294	WP_246535021.1	Txe/YoeB family addiction module toxin	-
KEF85_RS02330 (KEF85_02335)	493267..493872	+	606	WP_215583137.1	hypothetical protein	-
KEF85_RS02335 (KEF85_02340)	494127..494387	+	261	WP_215583138.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
KEF85_RS02340 (KEF85_02345)	494390..494680	+	291	WP_215584965.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KEF85_RS02345 (KEF85_02350)	495074..495568	+	495	WP_215583139.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KEF85_RS02350 (KEF85_02355)	495555..495977	+	423	WP_215583140.1	putative toxin-antitoxin system toxin component, PIN family	-
KEF85_RS02355 (KEF85_02360)	496465..496617	-	153	Protein_466	IS5/IS1182 family transposase	-
KEF85_RS02360 (KEF85_02365)	496784..497110	-	327	Protein_467	transposase domain-containing protein	-
KEF85_RS02365 (KEF85_02370)	497098..497328	-	231	WP_215579471.1	hypothetical protein	-
KEF85_RS02370 (KEF85_02375)	497374..497727	-	354	WP_215579475.1	IS66 family insertion sequence element accessory protein TnpB	-
KEF85_RS02375 (KEF85_02380)	497736..498065	-	330	WP_215579477.1	hypothetical protein	-
KEF85_RS02380 (KEF85_02385)	498286..498492	-	207	Protein_471	transposase domain-containing protein	-
KEF85_RS02385 (KEF85_02390)	498524..498736	+	213	Protein_472	transposase domain-containing protein	-
KEF85_RS02390 (KEF85_02395)	498823..499092	-	270	WP_215583143.1	DUF2442 domain-containing protein	-
KEF85_RS02395 (KEF85_02400)	499052..499291	-	240	WP_215583144.1	DUF4160 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-92)

Antitoxin

• Antitoxin ParD (7-76)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11523.20 Da
• Isoelectric Point: 6.7480

>T200153 WP_215584965.1 NZ_CP073754:494390-494680 [Methylomonas paludis]
MRKIHTQALAKQDIKNIWLYSFNKWGEEQADFYFDELNDAFALIAENPELGFACDYIREGYRQFHINRHLIFYCVSATKI
HIIRVLHDSMNYIVHL

Nucleotide

• Length: 291 bp

>T200153 NZ_CP097717:894-1043 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9663.84 Da
• Isoelectric Point: 4.4243

>AT200153 WP_215583138.1 NZ_CP073754:494127-494387 [Methylomonas paludis]
MAQTRTQTVSLGEYWNNLIDSLLKSGRYASVSEIMRDSLRLLEEREANSKLQSLRMALIEGEESGDSGELEMETIRQEAK
KEAGLA

Nucleotide

• Length: 261 bp

>AT200153 NZ_CP097717:c850-789 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT