Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | omega-epsilon-zeta/zeta-epsilon |
| Location | 3173773..3174205 | Replicon | chromosome |
| Accession | NZ_CP073752 | ||
| Organism | Clostridioides difficile strain 2015 | ||
Toxin (Protein)
| Gene name | zeta | Uniprot ID | R4ZD79 |
| Locus tag | KFK07_RS14560 | Protein ID | WP_003423072.1 |
| Coordinates | 3173773..3173931 (-) | Length | 53 a.a. |
Antitoxin (Protein)
| Gene name | epsilon | Uniprot ID | R2XCR7 |
| Locus tag | KFK07_RS14565 | Protein ID | WP_000301765.1 |
| Coordinates | 3173933..3174205 (-) | Length | 91 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KFK07_RS19345 | 3169336..3169569 | - | 234 | WP_235693514.1 | helix-turn-helix domain-containing protein | - |
| KFK07_RS14530 (KFK07_14530) | 3169988..3170236 | - | 249 | WP_002323370.1 | helix-turn-helix domain-containing protein | - |
| KFK07_RS14535 (KFK07_14535) | 3170243..3170659 | - | 417 | WP_003423075.1 | sigma factor-like helix-turn-helix DNA-binding protein | - |
| KFK07_RS14540 (KFK07_14540) | 3171107..3171985 | - | 879 | WP_003423074.1 | ParA family protein | - |
| KFK07_RS14545 (KFK07_14545) | 3172088..3172348 | - | 261 | Protein_2791 | topoisomerase DNA-binding C4 zinc finger domain-containing protein | - |
| KFK07_RS14550 (KFK07_14550) | 3172518..3173255 | - | 738 | WP_002292226.1 | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | - |
| KFK07_RS14555 (KFK07_14555) | 3173380..3173721 | - | 342 | Protein_2793 | AAA family ATPase | - |
| KFK07_RS14560 (KFK07_14560) | 3173773..3173931 | - | 159 | WP_003423072.1 | zeta toxin family protein | Toxin |
| KFK07_RS14565 (KFK07_14565) | 3173933..3174205 | - | 273 | WP_000301765.1 | antitoxin | Antitoxin |
| KFK07_RS14570 (KFK07_14570) | 3174222..3174437 | - | 216 | WP_002321610.1 | peptide-binding protein | - |
| KFK07_RS14575 (KFK07_14575) | 3174624..3174983 | + | 360 | WP_003423065.1 | helix-turn-helix transcriptional regulator | - |
| KFK07_RS14580 (KFK07_14580) | 3175091..3175279 | - | 189 | WP_009269672.1 | cysteine-rich KTR domain-containing protein | - |
| KFK07_RS14585 (KFK07_14585) | 3175484..3175675 | - | 192 | WP_003423063.1 | hypothetical protein | - |
| KFK07_RS14590 (KFK07_14590) | 3175688..3176332 | - | 645 | WP_003423062.1 | ABC-2 transporter permease | - |
| KFK07_RS14595 (KFK07_14595) | 3176329..3177192 | - | 864 | WP_003423060.1 | ABC transporter ATP-binding protein | - |
| KFK07_RS14600 (KFK07_14600) | 3177193..3177570 | - | 378 | WP_003423058.1 | GntR family transcriptional regulator | - |
| KFK07_RS14605 (KFK07_14605) | 3177691..3178602 | - | 912 | WP_003423057.1 | conjugal transfer protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | erm(B) | - | 3167872..3195228 | 27356 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 53 a.a. Molecular weight: 5776.53 Da Isoelectric Point: 10.3088
>T200152 WP_003423072.1 NZ_CP073752:c3173931-3173773 [Clostridioides difficile]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRRR
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRRR
Download Length: 159 bp
>T200152 NZ_CP097716:c4612606-4612433 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG
Antitoxin
Download Length: 91 a.a. Molecular weight: 10721.25 Da Isoelectric Point: 6.4676
>AT200152 WP_000301765.1 NZ_CP073752:c3174205-3173933 [Clostridioides difficile]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA
Download Length: 273 bp
>AT200152 NZ_CP097716:c4612930-4612634 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R4ZD79 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| PDB | 3Q8X | |
| PDB | 1GVN | |
| AlphaFold DB | A0A829F0A3 |