Detailed information of TA system TA020015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 479544..480162
• Replicon: chromosome
• Accession: CP009789
• Organism: Escherichia coli K-12 strain ER3413

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: ER3413_471
• Protein ID: AIZ54311.1
• Coordinates: 479544..479762 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: ER3413_472
• Protein ID: AIZ54312.1
• Coordinates: 479788..480162 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER3413_465	474833..475405	+	573	AIZ54304.1	outer membrane lipoprotein	-
ER3413_466	475436..475825	-	390	AIZ54305.1	excision repair protein, alkyltransferase-like protein ATL	-
ER3413_467	476126..476479	+	354	AIZ54306.1	conserved protein, DUF1428 family	-
ER3413_468	476521..478071	-	1551	AIZ54307.1	putative membrane-anchored cyclic-di-GMP phosphodiesterase	-
ER3413_469	478235..478705	-	471	AIZ54308.1	inner membrane protein, DUF1449 family	-
ER3413_470	478821..479372	-	552	AIZ54310.1	maltose O-acetyltransferase	-
ER3413_471	479544..479762	-	219	AIZ54311.1	modulator of gene expression, with H-NS	Toxin
ER3413_472	479788..480162	-	375	AIZ54312.1	Hha toxicity attenuator; conjugation-related protein	Antitoxin
ER3413_473	480708..483857	-	3150	AIZ54313.1	multidrug efflux system protein	-
ER3413_474	483880..485073	-	1194	AIZ54314.1	multidrug efflux system	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T20015 AIZ54311.1 CP009789:c479762-479544 [Escherichia coli K-12]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T20015 CP009789:c479762-479544 [Escherichia coli K-12]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT20015 AIZ54312.1 CP009789:c480162-479788 [Escherichia coli K-12]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT20015 CP009789:c480162-479788 [Escherichia coli K-12]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG