Detailed information of TA system TA200144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1767815..1769574
• Replicon: chromosome
• Accession: NZ_CP073741
• Organism: Burkholderia pseudomallei strain AW9

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q63JY0
• Locus tag: KEX42_RS24590
• Protein ID: WP_004188815.1
• Coordinates: 1768222..1769574 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: C4I768
• Locus tag: KEX42_RS24585
• Protein ID: WP_004536731.1
• Coordinates: 1767815..1768225 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEX42_RS24575 (KEX42_24575)	1764352..1766748	-	2397	WP_045588572.1	cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB	-
KEX42_RS24580 (KEX42_24580)	1767214..1767477	+	264	WP_004199789.1	hypothetical protein	-
KEX42_RS24585 (KEX42_24585)	1767815..1768225	+	411	WP_004536731.1	helix-turn-helix domain-containing protein	Antitoxin
KEX42_RS24590 (KEX42_24590)	1768222..1769574	+	1353	WP_004188815.1	type II toxin-antitoxin system HipA family toxin	Toxin
KEX42_RS24595 (KEX42_24595)	1769731..1770183	-	453	WP_004198842.1	hypothetical protein	-
KEX42_RS24600 (KEX42_24600)	1770284..1770430	-	147	WP_004199790.1	hypothetical protein	-
KEX42_RS24605 (KEX42_24605)	1770600..1771514	-	915	WP_004198844.1	LysR family transcriptional regulator	-
KEX42_RS24610 (KEX42_24610)	1771681..1772634	+	954	WP_004187785.1	agmatinase	-
KEX42_RS24615 (KEX42_24615)	1772905..1773246	+	342	WP_004539420.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-408)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-71)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49848.83 Da
• Isoelectric Point: 9.6807

>T200144 WP_004188815.1 NZ_CP073741:1768222-1769574 [Burkholderia pseudomallei]
VSARRARATRLHLWMNGLPVGYWEHARDGERLVYFDEWIGDPQGRPLSLSLPFTPGNQPYRGRLVSDYFDNLLPDSEPIR
RRIAMRYRTGGTSAFALLATLGRDCVGALQMLPPDEAPDDIERIRGHALADADIARLLREVTSAPQAGRHAPLDDLRLSI
AGAQEKTALLRHRGRWLLPEGSTPTTHILKLPLGLVGNRRADMRTSVENEWLCARIVAAYGLPVARCDIAQFDDQKALVV
ERFDRRPSRDARWLLRLPQEDMCQATGTSALDKYQADGGPGIETIMEVLAGSEHARDDRRAFFAAQIVFWLLAATDGHAK
NFSIAHLPGNRYRSTPLYDVLSAHPVIGRGANQLPAQRARLAMGVRGKHIHYPLHQIRRRHWIAQGQRVGFAPADVDALI
DTLTARTADVVDAVSARLPRDFPRDVADAIFSGMLGLSARLAGDAAARAP

Nucleotide

• Length: 1353 bp

>T200144 NZ_CP097716:3718846-3719064 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14508.87 Da
• Isoelectric Point: 11.8540

>AT200144 WP_004536731.1 NZ_CP073741:1767815-1768225 [Burkholderia pseudomallei]
MAILIEHEIKTLGQLRPILRGFRKSAGLTQAMLASRLGVTQQTYAQFEANPASASVERLFKVLRALDIELTLTLTQVYAA
PAGKDKGEVAKTAAGARARAGARRAVPPASAPAPSAAGRAPRPARKRAAPKKREDW

Nucleotide

• Length: 411 bp

>AT200144 NZ_CP097716:3718446-3718820 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A3N4FSX2

Antitoxin

• AlphaFold DB: A0A7V8KZI9